Dataset Information

Nano-graphene oxide with antisense walR RNA inhibits the pathogenicity of Enterococcus faecalis in periapical periodontitis.

ABSTRACT:

Background/purpose

Enterococcus faecalis (E. faecalis) is considered a predominant pathogen for persistent periapical infections. Antisense walR (ASwalR) RNA was reported to inhibit the biofilm formation and sensitized E. faecalis to calcium hydroxide medication. The aims of this study were to investigate whether the graphene oxide (GO) nanosheets could be used to enhance antibacterial activity of ASwalR RNA for E. faecalis in periapical periodontitis.

Materials and methods

We developed a graphene-based plasmid transformation system by loading antisense walR plasmid with GO-polyethylenimine (PEI) complexes (GO-PEI-ASwalR). The particle size distributions and zeta-potential of the GO-PEI-ASwalR were evaluated. Then, ASwalR plasmids were labeled with gene encoding enhanced green fluorescent protein (ASwalR-eGFP). The transformation efficiencies and the bacterial viability of E. faecalis were evaluated by confocal laser scanning microscopy. Quantitative real-time PCR assays were used to investigate the expressions of E. faecalis virulent genes after transformed by GO-PEI-ASwalR. Also, the antibacterial properties of the GO-PEI-ASwalR were validated in the rat periapical periodontitis model.

Results

We showed that GO-PEI could efficiently deliver the ASwalR plasmid into E. faecalis cell. GO-PEI-ASwalR significantly reduced virulent-associated gene expressions. Furthermore, GO-PEI-ASwalR suppressed biofilm aggregation and improved bactericidal effects using infected canal models in vitro. In four-weeks periapical infective rat models, the GO-PEI-ASwalR strains remarkably reduced the periapical lesion size.

Conclusion

Transformation efficiency and antibacterial prosperity of ASwalR can be marked improved by GO-PEI based delivery system for E. faecalis infections.

SUBMITTER: Wu S

PROVIDER: S-EPMC7109485 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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Publications

Nano-graphene oxide with antisense walR RNA inhibits the pathogenicity of Enterococcus faecalis in periapical periodontitis.

Wu Shizhou S Liu Yunjie Y Zhang Hui H Lei Lei L

Journal of dental sciences 20191023 1

<h4>Background/purpose</h4>Enterococcus faecalis (E. faecalis) is considered a predominant pathogen for persistent periapical infections. Antisense walR (ASwalR) RNA was reported to inhibit the biofilm formation and sensitized E. faecalis to calcium hydroxide medication. The aims of this study were to investigate whether the graphene oxide (GO) nanosheets could be used to enhance antibacterial activity of ASwalR RNA for E. faecalis in periapical p ...[more]

PMID: 32257002

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Project description:Enterococci are the third leading cause of hospital associated infections and have gained increased importance due to their fast adaptation to the clinical environment by acquisition of antibiotic resistance and pathogenicity traits. Enterococcus faecalis harbours a pathogenicity island (PAI) of 153 kb containing several virulence factors including the enterococcal surface protein (esp). Until now only internal fragments of the PAI or larger chromosomal regions containing it have been transferred. Here we demonstrate precise excision, circularization and horizontal transfer of the entire PAI element from the chromosome of E. faecalis strain UW3114. This PAI (ca. 200 kb) contained some deletions and insertions as compared to the PAI of the reference strain MMH594, transferred precisely and integrated site-specifically into the chromosome of E. faecalis (intergenic region) and Enterococcus faecium (tRNAlys). The internal PAI structure was maintained after transfer. We assessed phenotypic changes accompanying acquisition of the PAI and expression of some of its determinants. The esp gene is expressed on the surface of donor and both transconjugants. Biofilm formation and cytolytic activity were enhanced in E. faecalis transconjugants after acquisition of the PAI. No differences in pathogenicity of E. faecalis were detected using a mouse bacteraemia and a mouse peritonitis models (tail vein and intraperitoneal injection). A 66 kb conjugative pheromone-responsive plasmid encoding erm(B) (pLG2) that was transferred in parallel with the PAI was sequenced. pLG2 is a pheromone responsive plasmid that probably promotes the PAI horizontal transfer, encodes antibiotic resistance features and contains complete replication and conjugation modules of enterococcal origin in a mosaic-like composition. The E. faecalis PAI can undergo precise intra- and interspecies transfer probably with the help of conjugative elements like conjugative resistance plasmids, supporting the role of horizontal gene transfer and antibiotic selective pressure in the successful establishment of certain enterococci as nosocomial pathogens.

Dataset Information

Nano-graphene oxide with antisense walR RNA inhibits the pathogenicity of Enterococcus faecalis in periapical periodontitis.

Background/purpose

Materials and methods

Results

Conclusion

Publications

Nano-graphene oxide with antisense <i>walR</i> RNA inhibits the pathogenicity of <i>Enterococcus faecalis</i> in periapical periodontitis.

Similar Datasets

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