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Production and characterization of neutralizing monoclonal antibodies that recognize an epitope in domain 2 of Bacillus anthracis protective antigen.

ABSTRACT: Antibodies against the protective antigen (PA) of Bacillus anthracis play a key role in response to infection by this important pathogen. The aim of this study was to produce and characterize monoclonal antibodies (mAbs) specific for PA and to identify novel neutralizing epitopes. Three murine mAbs with high specificity and nanomolar affinity for B. anthracis recombinant protective antigen (rPA) were produced and characterized. Western immunoblot analysis, coupled with epitope mapping using overlapping synthetic peptides, revealed that these mAbs recognize a linear epitope within domain 2 of rPA. Neutralization assays demonstrate that these mAbs effectively neutralize lethal toxin in vitro.

SUBMITTER: Gubbins MJ

PROVIDER: S-EPMC7110350 | biostudies-literature | 2006 Aug

REPOSITORIES: biostudies-literature

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Production and characterization of neutralizing monoclonal antibodies that recognize an epitope in domain 2 of Bacillus anthracis protective antigen.

Gubbins Michael J MJ Berry Jody D JD Corbett Cindi R CR Mogridge Jeremy J Yuan Xin Y XY Schmidt Lisa L Nicolas Brigitte B Kabani Amin A Tsang Raymond S RS

FEMS immunology and medical microbiology 20060801 3

Antibodies against the protective antigen (PA) of Bacillus anthracis play a key role in response to infection by this important pathogen. The aim of this study was to produce and characterize monoclonal antibodies (mAbs) specific for PA and to identify novel neutralizing epitopes. Three murine mAbs with high specificity and nanomolar affinity for B. anthracis recombinant protective antigen (rPA) were produced and characterized. Western immunoblot analysis, coupled with epitope mapping using over ...[more]

PMID: 16872381

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A monoclonal antibody to Bacillus anthracis protective antigen defines a neutralizing epitope in domain 1.

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