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Evaluation of rapid assays for the detection of bovine coronavirus, rotavirus A and Cryptosporidium parvum in faecal samples of calves.


ABSTRACT: Rapid immunochromatographic assays for detecting infections with bovine coronavirus (BCV), rotavirus A and Cryptosporidium parvum in calf faeces were evaluated using as gold standards a reverse transcriptase polymerase chain reaction (BCV and rotavirus) and a sedimentation-flotation technique (C. parvum). Rapid tests for the detection of BCV and rotavirus showed a high specificity (96.4% and 95.3%, respectively), but a relatively low sensitivity (60.0% and 71.9%, respectively). Sensitivity and specificity for detection of C. parvum were high (100% and 94.6%, respectively).

SUBMITTER: Klein D 

PROVIDER: S-EPMC7110451 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature

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Evaluation of rapid assays for the detection of bovine coronavirus, rotavirus A and Cryptosporidium parvum in faecal samples of calves.

Klein Daniela D   Kern Angela A   Lapan Gudrun G   Benetka Viviane V   Möstl Karin K   Hassl Andreas A   Baumgartner Walter W  

Veterinary journal (London, England : 1997) 20080907 3


Rapid immunochromatographic assays for detecting infections with bovine coronavirus (BCV), rotavirus A and Cryptosporidium parvum in calf faeces were evaluated using as gold standards a reverse transcriptase polymerase chain reaction (BCV and rotavirus) and a sedimentation-flotation technique (C. parvum). Rapid tests for the detection of BCV and rotavirus showed a high specificity (96.4% and 95.3%, respectively), but a relatively low sensitivity (60.0% and 71.9%, respectively). Sensitivity and s  ...[more]

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