Protective role of follicular CXCR5⁺CD8⁺ T cells against dengue virus 2 infection.

ABSTRACT: Follicular CXCR5⁺CD8⁺ T cells have antiviral effects in chronic virus infection, but the roles of these cells during dengue virus 2 (DENV2) infection remain poorly understood.

Objective

This study was conducted to analyzed in detail the dynamic changes and functional properties of circulating follicular CXCR5⁺CD8⁺ T cells to explore their effects on DENV2 infection.

Methods

Circulating follicular CXCR5⁺CD8⁺ T cells and cytokines were analyzed by flow cytometry in DENV2 patients at difference days after DENV2 infection. CD8⁺ T cells were isolated and purified from DENV2 patients, then were stimulated with NS1 peptides and TCR stimulant. After cultivation, multiple parameters were tested.

Results

(1) CXCR5⁺CD8⁺ T cells emerged after DENV2 infection, with high PD-1 expression, and were correlated with the reduction in DENV2 RNA viral loads. (2) PD-1⁺CXCR5⁺CD8⁺ T cells were negatively associated with disease progression. (3) Serum IFN-?, IL-6 and IL-10 levels were increased late in the course of DENV2 infection. (4) CXCR5⁺CD8⁺ T cells from DENV2 patients exhibited increased cytotoxicity and IFN-? and IL-10 secretion.

Conclusion

CXCR5⁺CD8⁺ T cells could play a protective role in dengue pathogenesis and may be a novel strategy for controlling DENV2 infection and vaccine development.

SUBMITTER: Qiu L 

PROVIDER: S-EPMC7110549 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature