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Virus purification and enrichment by hydroxyapatite chromatography on a chip.

ABSTRACT: The spread of infectious diseases has become a global health concern. In order to diagnose infectious diseases quickly and accurately, next-generation DNA sequencing techniques for genetic analysis of infectious viruses have been developed rapidly. However, it takes a very long time to pretreat clinical samples for genetic analysis using next-generation sequencers. We have therefore developed a microfluidic chromatography chip that can purify and enrich viruses in a sample using hydroxyapatite particles packed in a micro-column. We demonstrated the purification of virus from a mixture of virus and FBS protein, and enrichment of the virus using this novel microfluidic chip.

SUBMITTER: Niimi M

PROVIDER: S-EPMC7111472 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature

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Virus purification and enrichment by hydroxyapatite chromatography on a chip.

Niimi Miyako M Masuda Taisuke T Kaihatsu Kunihiro K Kato Nobuo N Nakamura Shota S Nakaya Takaaki T Arai Fumihito F

Sensors and actuators. B, Chemical 20140506

The spread of infectious diseases has become a global health concern. In order to diagnose infectious diseases quickly and accurately, next-generation DNA sequencing techniques for genetic analysis of infectious viruses have been developed rapidly. However, it takes a very long time to pretreat clinical samples for genetic analysis using next-generation sequencers. We have therefore developed a microfluidic chromatography chip that can purify and enrich viruses in a sample using hydroxyapatite p ...[more]

PMID: 32288247

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