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Detection of virus particles resembling circovirus and porcine circovirus 2a (PCV2a) sequences in feces of dogs.

ABSTRACT: During routine electron microscopy of fecal samples from diarrheic dogs dated from 2000 virus particles resembling circovirus in shape and size were detected in two samples (V2177/00; V3374/00). Polymerase chain reaction (PCR) using primers specific for porcine circovirus type 2 (PCV2) amplified DNA recovered from both samples. Sequencing of PCR amplificates (V2177/00) obtained with PCV2-specific primer pairs revealed a genome size of 1768bp. The nucleotide sequence was highly similar (98% nucleotide identity) to the PCV2a reference sequence.

SUBMITTER: Herbst W

PROVIDER: S-EPMC7111833 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature

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Detection of virus particles resembling circovirus and porcine circovirus 2a (PCV2a) sequences in feces of dogs.

Herbst Werner W Willems Hermann H

Research in veterinary science 20170121

During routine electron microscopy of fecal samples from diarrheic dogs dated from 2000 virus particles resembling circovirus in shape and size were detected in two samples (V2177/00; V3374/00). Polymerase chain reaction (PCR) using primers specific for porcine circovirus type 2 (PCV2) amplified DNA recovered from both samples. Sequencing of PCR amplificates (V2177/00) obtained with PCV2-specific primer pairs revealed a genome size of 1768bp. The nucleotide sequence was highly similar (98% nucle ...[more]

PMID: 28135670

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