Ontology highlight
ABSTRACT:
SUBMITTER: Decaro N
PROVIDER: S-EPMC7112840 | biostudies-literature | 2008 Aug
REPOSITORIES: biostudies-literature
Decaro Nicola N Elia Gabriella G Campolo Marco M Desario Costantina C Mari Viviana V Radogna Arianna A Colaianni Maria Loredana ML Cirone Francesco F Tempesta Maria M Buonavoglia Canio C
Journal of virological methods 20080624 2
A real-time reverse transcriptase-polymerase chain reaction (RT-PCR) for the detection of bovine coronavirus (BCoV) RNA in clinical samples is described. The assay is based on TaqMan technology, consisting of two primers and one probe labeled with the reporter dye 6-carboxyfluorescein that binds selectively to the transmembrane-protein gene of BCoV. The BCoV real-time RT-PCR assay was able to detect the tested BCoV and BCoV-like viruses (canine respiratory coronavirus and bubaline coronavirus), ...[more]