Ontology highlight
ABSTRACT:
SUBMITTER: Yongkiettrakul S
PROVIDER: S-EPMC7112848 | biostudies-literature | 2009 Mar
REPOSITORIES: biostudies-literature
Yongkiettrakul S S Boonyapakron K K Jongkaewwattana A A Wanitchang A A Leartsakulpanich U U Chitnumsub P P Eurwilaichitr L L Yuthavong Y Y
Journal of virological methods 20081211 1-2
The study reports heterologous expression in Pichia pastoris of active neuraminidase derived from avian influenza virus A/Viet Nam/DT-036/2005(H5N1). A gene encoding the neuraminidase N1 head domain (residues 63-449) was fused directly in-frame with the Saccharomyces cerevisiae alpha-factor secretion signal in pPICZ(A vector. Recombinant N1 neuraminidase was expressed in P. pastoris as a 72kDa secreted, soluble protein. Glycopeptidase F treatment generated a 45kDa product, indicating that the se ...[more]