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A reverse transcription loop-mediated isothermal amplification method for rapid detection of bovine viral diarrhea virus.

ABSTRACT: A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed and optimized to detect bovine viral diarrhea viral (BVDV) RNA. The RT-LAMP assay is highly sensitive and able to detect 4.67×10(0)copies of BVDV RNA. Additionally, the RT-LAMP method is capable of detecting both genotypes of BVDV. No cross-reaction with other bovine viruses was observed. The ability of RT-LAMP to detect BVDV RNA from bovine fecal swabs was also evaluated. Of the 88 fecal swabs, 38 were found to be positive by RT-LAMP assay, whereas 39 were positive by real-time RT-PCR. Taken together, the BVDV specific RT-LAMP method is highly specific and sensitive and can be used as a rapid and direct diagnostic assay for testing clinical samples.

SUBMITTER: Fan Q

PROVIDER: S-EPMC7112856 | biostudies-literature | 2012 Dec

REPOSITORIES: biostudies-literature

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A reverse transcription loop-mediated isothermal amplification method for rapid detection of bovine viral diarrhea virus.

Fan Qing Q Xie Zhixun Z Xie Liji L Liu Jiabo J Pang Yaoshan Y Deng Xianwen X Xie Zhiqin Z Peng Yi Y Wang Xiuqing X

Journal of virological methods 20120827 1-2

A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed and optimized to detect bovine viral diarrhea viral (BVDV) RNA. The RT-LAMP assay is highly sensitive and able to detect 4.67×10(0)copies of BVDV RNA. Additionally, the RT-LAMP method is capable of detecting both genotypes of BVDV. No cross-reaction with other bovine viruses was observed. The ability of RT-LAMP to detect BVDV RNA from bovine fecal swabs was also evaluated. Of the 88 fecal swabs, 38 were ...[more]

PMID: 22947692

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