Ontology highlight
ABSTRACT:
SUBMITTER: Fan Q
PROVIDER: S-EPMC7112856 | biostudies-literature | 2012 Dec
REPOSITORIES: biostudies-literature
Journal of virological methods 20120827 1-2
A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed and optimized to detect bovine viral diarrhea viral (BVDV) RNA. The RT-LAMP assay is highly sensitive and able to detect 4.67×10(0)copies of BVDV RNA. Additionally, the RT-LAMP method is capable of detecting both genotypes of BVDV. No cross-reaction with other bovine viruses was observed. The ability of RT-LAMP to detect BVDV RNA from bovine fecal swabs was also evaluated. Of the 88 fecal swabs, 38 were ...[more]