Ontology highlight
ABSTRACT:
SUBMITTER: Mourez T
PROVIDER: S-EPMC7112889 | biostudies-literature | 2007 Feb
REPOSITORIES: biostudies-literature
Mourez Thomas T Vabret Astrid A Han Yang Y Dina Julia J Legrand Loïc L Corbet Sandrine S Freymuth François F
Journal of virological methods 20061031 2
The nucleocapsid (N) gene of human coronavirus strain OC43 (HCoV-OC43) was amplified by reverse transcriptase-polymerase chain reaction, and cloned in pENTR/D-TOPO plasmid. This plasmid containing the N gene was recombined with in a BaculoDirect baculovirus DNA designed in order to express N protein in fusion with a C-terminal polyhistidine tag containing V5 epitope. Sf21 cells were transfected with recombinant baculovirus DNA. Recombinant N protein was extracted from infected cells, analysed by ...[more]