Ontology highlight
ABSTRACT:
SUBMITTER: Nogales A
PROVIDER: S-EPMC7112926 | biostudies-literature | 2011 Jul
REPOSITORIES: biostudies-literature
Nogales Aitor A Galán Carmen C Márquez-Jurado Silvia S García-Gallo Mónica M Kremer Leonor L Enjuanes Luis L Almazán Fernando F
Journal of virological methods 20110412 1
Coronavirus RNA synthesis is a sophisticated process performed by a viral multienzymatic replicase complex, together with cellular factors. A key enzyme of this replication complex is the RNA dependent RNA polymerase (RdRp). To study the replication of coronavirus genome, six monoclonal antibodies (mAbs) specific for transmissible gastroenteritis virus (TGEV) RdRp were generated and characterized. His-tagged RdRp was expressed in baculovirus, purified and used as immunogen to produce mAbs. The T ...[more]