Unknown

Dataset Information

0

The translational landscape of ground state pluripotency.


ABSTRACT: Translational control plays a central role in regulation of gene expression and can lead to significant divergence between mRNA- and protein-abundance. Here, we used genome-wide approaches combined with time-course analysis to measure the mRNA-abundance, mRNA-translation rate and protein expression during the transition of naïve-to-primed mouse embryonic stem cells (ESCs). We find that the ground state ESCs cultured with GSK3-, MEK-inhibitors and LIF (2iL) display higher ribosome density on a selective set of mRNAs. This set of mRNAs undergo strong translational buffering to maintain stable protein expression levels in 2iL-ESCs. Importantly, we show that the global alteration of cellular proteome during the transition of naïve-to-primed pluripotency is largely accompanied by transcriptional rewiring. Thus, we provide a comprehensive and detailed overview of the global changes in gene expression in different states of ESCs and dissect the relative contributions of mRNA-transcription, translation and regulation of protein stability in controlling protein abundance.

SUBMITTER: Atlasi Y 

PROVIDER: S-EPMC7113317 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

altmetric image

Publications

The translational landscape of ground state pluripotency.

Atlasi Yaser Y   Jafarnejad Seyed Mehdi SM   Gkogkas Christos G CG   Vermeulen Michiel M   Sonenberg Nahum N   Stunnenberg Hendrik G HG  

Nature communications 20200401 1


Translational control plays a central role in regulation of gene expression and can lead to significant divergence between mRNA- and protein-abundance. Here, we used genome-wide approaches combined with time-course analysis to measure the mRNA-abundance, mRNA-translation rate and protein expression during the transition of naïve-to-primed mouse embryonic stem cells (ESCs). We find that the ground state ESCs cultured with GSK3-, MEK-inhibitors and LIF (2iL) display higher ribosome density on a se  ...[more]

Similar Datasets

2020-03-01 | GSE133794 | GEO
2020-03-01 | GSE133792 | GEO
2020-03-01 | GSE133793 | GEO
| PRJNA552550 | ENA
| PRJNA552553 | ENA
| PRJNA552552 | ENA
| S-EPMC4680864 | biostudies-other
2020-04-03 | PXD014528 | Pride
| S-EPMC3398752 | biostudies-literature
| S-EPMC2685927 | biostudies-literature