Oropouche virus entry into HeLa cells involves clathrin and requires endosomal acidification.
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ABSTRACT:
SUBMITTER: Santos RI
PROVIDER: S-EPMC7114418 | biostudies-literature | 2008 Dec
REPOSITORIES: biostudies-literature
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Oropouche virus entry into HeLa cells involves clathrin and requires endosomal acidification.
Santos Rodrigo I M RI Rodrigues Alcir H AH Silva Maria Lúcia ML Mortara Renato A RA Rossi Marcos A MA Jamur Maria Célia MC Oliver Constance C Arruda Eurico E
Virus research 20081028 1-2
Oropouche virus (ORO), family Bunyaviridae, is the second most frequent cause of arboviral febrile illness in Brazil. Studies were conducted to understand ORO entry in HeLa cells. Chlorpromazine inhibited early steps of ORO replication cycle, consistent with entry/uncoating. The data indicate that ORO enters HeLa cells by clathrin-coated vesicles, by a mechanism susceptible to endosomal acidification inhibitors. Transmission electron microscopy and immunofluorescence indicated that ORO associate ...[more]
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