Preparation and characterization of SARS in-house reference antiserum.
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ABSTRACT:
SUBMITTER: Zhang J
PROVIDER: S-EPMC7115399 | biostudies-literature | 2005 Dec
REPOSITORIES: biostudies-literature
Publications
Preparation and characterization of SARS in-house reference antiserum.
Zhang Jiansan J Liu Yuxuan Y Hu Liangxiang L Gao Qiang Q Zhang Zhenshan Z Zhang Xiaomei X Chen Jiangting J Gong Xuejie X Song Lifei L Liu Yufen Y Li Jing J Li Shufen S Huang Jinfeng J Ning Ye Y Gao Hong H Qin Chuan C Dong Xiaoping X Wei Jianguo J Dong Guanmu G Yin Weidong W
Vaccine 20050316 48-49
A reference antiserum for SARS is in urgent need in the development of SARS vaccine and other serological test of SARS research. Convalescent serum was collected from clinical confirmed patient. ELISA, Western-blotting and neutralization assay detected specific antibody against SARS coronavirus. This antiserum was prepared as in-house reference antiserum, freeze-dried and sealed in ampoules. The potency of this reference antiserum is defined to be 52.7 U after extensive calibration. Further, col ...[more]
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