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Immune responses in Balb/c mice induced by a candidate SARS-CoV inactivated vaccine prepared from F69 strain.

ABSTRACT: The immunogenicity of a candidate-inactivated vaccine prepared from SARS-CoV F69 strain was evaluated in Balb/c mice. Potent humoral immune responses were induced under the elicitation of three times of immunizations at 2-week intervals with this vaccine, combined with three types of adjuvants (Freund's adjuvant, Al(OH)(3) adjuvant and CpG adjuvant). Titers of specific IgG antibodies in three test groups all peaked in the sixth week after first vaccination, but significant differences existed in the kinetics of specific IgG antibody levels. The strong neutralizing capacity exhibited in micro-cytopathic effect neutralization tests indicated the specific antibodies are protective. Western blot assay further demonstrated the specificity of the induced serum antibodies.

SUBMITTER: Zhang CH

PROVIDER: S-EPMC7115646 | biostudies-literature | 2005 May

REPOSITORIES: biostudies-literature

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Immune responses in Balb/c mice induced by a candidate SARS-CoV inactivated vaccine prepared from F69 strain.

Zhang Chuan-hai CH Lu Jia-hai JH Wang Yi-fei YF Zheng Huan-ying HY Xiong Sheng S Zhang Mei-ying MY Liu Xin-jian XJ Li Jiu-xiang JX Wan Zhuo-yue ZY Yan Xin-ge XG Qi Shu-Yuan SY Cui Zhiyong Z Zhang Biliang B

Vaccine 20050501 24

The immunogenicity of a candidate-inactivated vaccine prepared from SARS-CoV F69 strain was evaluated in Balb/c mice. Potent humoral immune responses were induced under the elicitation of three times of immunizations at 2-week intervals with this vaccine, combined with three types of adjuvants (Freund's adjuvant, Al(OH)(3) adjuvant and CpG adjuvant). Titers of specific IgG antibodies in three test groups all peaked in the sixth week after first vaccination, but significant differences existed in ...[more]

PMID: 15837220

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