Ontology highlight
ABSTRACT:
SUBMITTER: Cervettini D
PROVIDER: S-EPMC7116527 | biostudies-literature | 2020 Aug
REPOSITORIES: biostudies-literature
Cervettini Daniele D Tang Shan S Fried Stephen D SD Willis Julian C W JCW Funke Louise F H LFH Colwell Lucy J LJ Chin Jason W JW
Nature biotechnology 20200413 8
A central challenge in expanding the genetic code of cells to incorporate noncanonical amino acids into proteins is the scalable discovery of aminoacyl-tRNA synthetase (aaRS)-tRNA pairs that are orthogonal in their aminoacylation specificity. Here we computationally identify candidate orthogonal tRNAs from millions of sequences and develop a rapid, scalable approach-named tRNA Extension (tREX)-to determine the in vivo aminoacylation status of tRNAs. Using tREX, we test 243 candidate tRNAs in Esc ...[more]