Ontology highlight
ABSTRACT:
SUBMITTER: Soliman H
PROVIDER: S-EPMC7117309 | biostudies-literature | 2006 May
REPOSITORIES: biostudies-literature
Veterinary microbiology 20051227 3-4
A one step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for detection of viral hemorrhagic septicaemia virus (VHS). A set of six primers were designed, based on the G-protein sequence of the VHS virus serotypes (He, F1, 23.75, Klapmolle and Rindsholm). The assay was optimised to amplify VHS RNA by incubation at 63 degrees C for only 1h, and required only a simple water bath or heating block to provide a constant temperature of 63 degrees C. RT-LAMP a ...[more]