Ontology highlight
ABSTRACT:
SUBMITTER: Suresh P
PROVIDER: S-EPMC7117910 | biostudies-literature | 2020 Mar
REPOSITORIES: biostudies-literature
Suresh Pooja P Long Alexandra F AF Dumont Sophie S
eLife 20200319
The spindle generates force to segregate chromosomes at cell division. In mammalian cells, kinetochore-fibers connect chromosomes to the spindle. The dynamic spindle anchors kinetochore-fibers in space and time to move chromosomes. Yet, how it does so remains poorly understood as we lack tools to directly challenge this anchorage. Here, we adapt microneedle manipulation to exert local forces on the spindle with spatiotemporal control. Pulling on kinetochore-fibers reveals the preservation of loc ...[more]