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A Chromosome-Scale Assembly of the Asian Honeybee Apis cerana Genome.


ABSTRACT: Apis cerana is one of the main honeybee species in artificial farming, which is widely distributed in Asian countries. The genome of A. cerana has been sequenced by several different research groups using second generation sequencing technologies. However, it is still necessary to obtain more complete and accurate genome sequences. Here we present a chromosome-scale assembly of the A. cerana genome using single-molecule real-time (SMRT) Pacific Biosciences sequencing and high-throughput chromatin conformation capture (Hi-C) genome scaffolding. The updated assembly is 215.67 Mb in size with a contig N50 of 4.49 Mb, representing an 212-fold improvement over the previous Illumina-based version. Hi-C scaffolding resulted in 16 pseudochromosomes occupying 97.85% of the assembled genome sequences. A total of 10,741 protein-coding genes were predicted and 9,627 genes were annotated. Besides, 314 new genes were identified compared to the previous version. The improved high-quality A. cerana reference genome will provide precise sequence information for biological research of A. cerana.

SUBMITTER: Wang ZL 

PROVIDER: S-EPMC7119468 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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A Chromosome-Scale Assembly of the Asian Honeybee <i>Apis cerana</i> Genome.

Wang Zi-Long ZL   Zhu Yong-Qiang YQ   Yan Qing Q   Yan Wei-Yu WY   Zheng Hua-Jun HJ   Zeng Zhi-Jiang ZJ  

Frontiers in genetics 20200327


<i>Apis cerana</i> is one of the main honeybee species in artificial farming, which is widely distributed in Asian countries. The genome of <i>A. cerana</i> has been sequenced by several different research groups using second generation sequencing technologies. However, it is still necessary to obtain more complete and accurate genome sequences. Here we present a chromosome-scale assembly of the <i>A. cerana</i> genome using single-molecule real-time (SMRT) Pacific Biosciences sequencing and hig  ...[more]

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