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A single tube RT-PCR assay for the detection of mosquito-borne flaviviruses.

ABSTRACT: Mosquito-borne flaviviruses include several important agents of human disease and have provided striking examples of emerging infections. In this study we present the design and validation of a single tube RT-PCR assay using a pair of consensus primers for the detection of mosquito-borne flaviviruses. Sequencing of the amplicons permits the species identification. The assay was validated using RNA from the yellow fever virus vaccine strain and from representative strains of dengue viruses 1, 2, 3 and 4, West Nile virus, Kunjin virus (a clade of West Nile virus), and St. Louis encephalitis virus.

SUBMITTER: Ayers M

PROVIDER: S-EPMC7119486 | biostudies-literature | 2006 Aug

REPOSITORIES: biostudies-literature

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A single tube RT-PCR assay for the detection of mosquito-borne flaviviruses.

Ayers M M Adachi D D Johnson G G Andonova M M Drebot M M Tellier R R

Journal of virological methods 20060502 2

Mosquito-borne flaviviruses include several important agents of human disease and have provided striking examples of emerging infections. In this study we present the design and validation of a single tube RT-PCR assay using a pair of consensus primers for the detection of mosquito-borne flaviviruses. Sequencing of the amplicons permits the species identification. The assay was validated using RNA from the yellow fever virus vaccine strain and from representative strains of dengue viruses 1, 2, ...[more]

PMID: 16650488

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