An LC-QToF MS based method for untargeted metabolomics of human fecal samples.
Ontology highlight
ABSTRACT: Introduction
Consensus in sample preparation for untargeted human fecal metabolomics is lacking.Objectives
To obtain sample preparation with broad metabolite coverage for high-throughput LC-MS.Methods
Extraction solvent, solvent ratio and fresh frozen-vs-lyophilized samples were evaluated by metabolite feature quality.Results
Methanol at 5 mL per g wet feces provided a wide metabolite coverage with optimal balance between signal intensity and saturation for both fresh frozen and lyophilized samples. Lyophilization did not affect SCFA and is recommended because of convenience in normalizing to dry matter.Conclusion
The suggested sample preparation is simple, efficient and suitable for large-scale human fecal metabolomics.
SUBMITTER: Cheng K
PROVIDER: S-EPMC7125068 | biostudies-literature | 2020 Apr
REPOSITORIES: biostudies-literature
Publications
An LC-QToF MS based method for untargeted metabolomics of human fecal samples.
Cheng Ken K Brunius Carl C Fristedt Rikard R Landberg Rikard R
Metabolomics : Official journal of the Metabolomic Society 20200403 4
<h4>Introduction</h4>Consensus in sample preparation for untargeted human fecal metabolomics is lacking.<h4>Objectives</h4>To obtain sample preparation with broad metabolite coverage for high-throughput LC-MS.<h4>Methods</h4>Extraction solvent, solvent ratio and fresh frozen-vs-lyophilized samples were evaluated by metabolite feature quality.<h4>Results</h4>Methanol at 5 mL per g wet feces provided a wide metabolite coverage with optimal balance between signal intensity and saturation for both f ...[more]
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