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Preparation and standardization of Mathan Tailam: A classical Siddha formulation for diabetic ulcerative wound healing.

ABSTRACT: BACKGROUND:Increasing popularity of Mathan Tailam (mattan tailam, pachai ennai) for the treatment of diabetic foot ulcer necessitated standardization and quality control of this medicated oil both in large scale production and quality check in marketed drug. OBJECTIVE:Present study aims to develop standard operating procedure for the preparation of Mathan Tailam as per Siddha Formulary of India and its standardization using suitable analytical techniques. MATERIALS AND METHODS:Mathan Tailam was prepared as per Siddha Formulary of India. Physicochemical parameters and preliminary phytochemical screening were carried out using standard methods. The in-house prepared sample underwent physico-chemical analysis, qualitative phytochemical analysis, Gas chromatography-mass spectrum (GC-MS) analysis, High performance thin layer chromatographic (HPTLC) fingerprinting profile and inductively coupled plasma-optical emission spectroscopic (ICP-OES) analysis. RESULTS:Physico-chemical parameters of the prepared formulation were comparable to that of coconut oil. Aqueous methonolic extract of this drug was found to be positive for alkaloid, saponin, coumarin, steroid, triterpinoid, quinine and furan. The GC-MS values were comparable to that of the base used i.e., the coconut oil. HPTLC fingerprinting profile revealed the presence of phytochemicals in the medicated oil derived from both coconut oil and Datura metel. ICP-OES addressed the mineral portion of the formulation and its safety in heavy metal aspect. CONCLUSION:All these parameters can be utilized for the overall quality check over its preparation and formulation.

SUBMITTER: Arunadevi R

PROVIDER: S-EPMC7125365 | biostudies-literature | 2020 Jan - Mar

REPOSITORIES: biostudies-literature

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Preparation and standardization of Mathan Tailam: A classical Siddha formulation for diabetic ulcerative wound healing.

Arunadevi R R Susila R R Murugammal S S Divya S S

Journal of Ayurveda and integrative medicine 20180316 1

<h4>Background</h4>Increasing popularity of Mathan Tailam (mattan tailam, pachai ennai) for the treatment of diabetic foot ulcer necessitated standardization and quality control of this medicated oil both in large scale production and quality check in marketed drug.<h4>Objective</h4>Present study aims to develop standard operating procedure for the preparation of Mathan Tailam as per Siddha Formulary of India and its standardization using suitable analytical techniques.<h4>Materials and methods< ...[more]

PMID: 29555256

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Project description:BackgroundWe recently reported the clinical safety profile of RJX, a well-defined intravenous GMP-grade pharmaceutical formulation of anti-oxidant and anti-inflammatory vitamins as active ingredients, in a Phase 1 study in healthy volunteers (ClinicalTrials.gov Identifier: NCT03680105) (Uckun et al., Front. Pharmacol. 11, 594321. 10.3389/fphar.2020.594321). The primary objective of the present study was to examine the effects of GMP-grade RJX on wound and burn injury healing in diabetic rats.MethodsIn the present study, a rat model of T2DM was used that employs HFD in combination with a single injection of STZ intraperitoneally (i.p) at a moderate dose level (45 mg/kg). Anesthetized diabetic rats underwent full-thickness skin excision on the back or were subjected to burn injury via a heated brass probe and then started on treatments with normal saline (NS = vehicle) or RJX administered via intraperitoneal injections for three weeks.FindingsNotably, diabetic rats treated with the 1.25 mL/kg or 2.5 mL/kg RJX (DM+RJX groups) rapidly healed their wounds as fast as non-diabetic control rats. Inflammatory cell infiltration in the dermis along with fibrin and cell debris on the epithelial layer persisted for up to 14 days in the DM+NS group but not in RJX-treated groups. The histopathological score of wound healing on days 7 and 14 was better in diabetic rats treated with RJX than diabetic rats treated with NS and comparable to the scores for non-diabetic healthy rats consistent with an accelerated healing process. The residual wound area of RJX-treated rats was significantly smaller than that of NS-treated diabetic rats at each evaluation time point (P<0.001). The accelerating effect of RJX on diabetic wound healing was dose-dependent. We obtained similar results in the burn injury model. Our results demonstrate that RJX - at a dose level >10-fold lower than its clinical maximum tolerated dose (MTD) - accelerates the healing of excision wounds as well burn injury in diabetic rats.

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Preparation and standardization of Mathan Tailam: A classical Siddha formulation for diabetic ulcerative wound healing.

Publications

Preparation and standardization of Mathan Tailam: A classical Siddha formulation for diabetic ulcerative wound healing.

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