CRISPR/dCas9-mediated biosensor for detection of tick-borne diseases.
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ABSTRACT:
SUBMITTER: Koo B
PROVIDER: S-EPMC7126152 | biostudies-literature | 2018 Nov
REPOSITORIES: biostudies-literature
Publications
CRISPR/dCas9-mediated biosensor for detection of tick-borne diseases.
Koo Bonhan B Kim Da-Eun DE Kweon Jiyeon J Jin Choong Eun CE Kim Sung-Han SH Kim Yongsub Y Shin Yong Y
Sensors and actuators. B, Chemical 20180615
Rapid and highly sensitive detection of biomolecules is greatly needed for pathogen diagnosis in clinical samples, but the method needs to be significantly improved in terms of sensitivity and specificity for actual use in clinical settings. Here, we report the development of an improved molecular diagnostics tool that utilizes CRISPR/dCas9-mediated biosensor that couples a nuclease inactivated Cas9 (dCas9) and single microring resonator biosensor, enables label-free and real-time detection of p ...[more]
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