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MultiBac: expanding the research toolbox for multiprotein complexes.

ABSTRACT: Protein complexes composed of many subunits carry out most essential processes in cells and, therefore, have become the focus of intense research. However, deciphering the structure and function of these multiprotein assemblies imposes the challenging task of producing them in sufficient quality and quantity. To overcome this bottleneck, powerful recombinant expression technologies are being developed. In this review, we describe the use of one of these technologies, MultiBac, a baculovirus expression vector system that is particularly tailored for the production of eukaryotic multiprotein complexes. Among other applications, MultiBac has been used to produce many important proteins and their complexes for their structural characterization, revealing fundamental cellular mechanisms.

SUBMITTER: Bieniossek C

PROVIDER: S-EPMC7127121 | biostudies-literature | 2012 Feb

REPOSITORIES: biostudies-literature

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MultiBac: expanding the research toolbox for multiprotein complexes.

Bieniossek Christoph C Imasaki Tsuyoshi T Takagi Yuichiro Y Berger Imre I

Trends in biochemical sciences 20111207 2

Protein complexes composed of many subunits carry out most essential processes in cells and, therefore, have become the focus of intense research. However, deciphering the structure and function of these multiprotein assemblies imposes the challenging task of producing them in sufficient quality and quantity. To overcome this bottleneck, powerful recombinant expression technologies are being developed. In this review, we describe the use of one of these technologies, MultiBac, a baculovirus expr ...[more]

PMID: 22154230

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