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Direct and label-free influenza virus detection based on multisite binding to sialic acid receptors.

ABSTRACT: A system to discriminate human or avian influenza A remains a highly sought-after tool for prevention of influenza pandemics in humans. Selective binding of the influenza A viral hemagglutinin (HA) to specific sialic acid (SA) receptors (Neu5Ac?(2-6)Gal in humans, Neu5Ac?(2-3)Gal in birds) is determined by the genotype of the HA and neuraminidase (NA) segments, making it one of the key characteristics that distinguishes human or avian influenza A virus. Here we demonstrate the direct detection of whole H1N1 influenza A virus using 6'-sialyllactose (Neu5Ac?(2-6)Gal?(1-4)Glc, 6SL)-immobilized gold electrodes as biosensing surfaces. The sensitivity was higher than that of conventional immunochromatographic technique (ICT) for influenza virus and not restricted by genetic drift. The label-free detection technology via direct attachment of a whole virus using a chemically modified electrode is a promising means to provide a simple and rapid diagnostic system for viral infections.

SUBMITTER: Horiguchi Y 

PROVIDER: S-EPMC7127512 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

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Direct and label-free influenza virus detection based on multisite binding to sialic acid receptors.

Horiguchi Yukichi Y   Goda Tatsuro T   Matsumoto Akira A   Takeuchi Hiroaki H   Yamaoka Shoji S   Miyahara Yuji Y  

Biosensors & bioelectronics 20170216

A system to discriminate human or avian influenza A remains a highly sought-after tool for prevention of influenza pandemics in humans. Selective binding of the influenza A viral hemagglutinin (HA) to specific sialic acid (SA) receptors (Neu5Acα(2-6)Gal in humans, Neu5Acα(2-3)Gal in birds) is determined by the genotype of the HA and neuraminidase (NA) segments, making it one of the key characteristics that distinguishes human or avian influenza A virus. Here we demonstrate the direct detection o  ...[more]

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