Ontology highlight
ABSTRACT:
SUBMITTER: Sekiya M
PROVIDER: S-EPMC7127579 | biostudies-literature | 2006 Sep
REPOSITORIES: biostudies-literature
Sekiya Mary M Mulcahy Grace G Irwin Jane A JA Stack Colin M CM Donnelly Sheila M SM Xu Weibo W Collins Peter P Dalton John P JP
FEBS letters 20060822 21
The parasitic helminth Fasciola hepatica secretes a 2-Cys peroxiredoxin (Prx) that may play important functions in host-parasite interaction. Recombinant peroxiredoxin (FhePrx) prevented metal-catalyzed oxidative nicking of plasmid DNA and detoxified hydrogen peroxide when coupled with Escherichia coli thioredoxin and thioredoxin reductase (k(cat)/K(m)=5.2 x 10(5)M(-1)s(-1)). Enzyme kinetic analysis revealed that the catalytic efficiency of FhePrx is similar to other 2-Cys peroxiredoxins; the en ...[more]