Establishment of a stable CHO cell line with high level expression of recombinant porcine IFN-?.
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ABSTRACT:
SUBMITTER: Chen W
PROVIDER: S-EPMC7128424 | biostudies-literature | 2011 Jun
REPOSITORIES: biostudies-literature
Publications
Establishment of a stable CHO cell line with high level expression of recombinant porcine IFN-β.
Chen Weiye W Cao Wenyan W Zhao Huijun H Hu Qianqian Q Qu Linmao L Hu Sen S Ge Jinying J Wen Zhiyuan Z Wang Xijun X Li Haobo H Huang Kehe K Bu Zhigao Z
Cytokine 20110401 3
A CHO cell clone (CHO-PoIFN-β) with stable porcine IFN-β expression under control of CMV promoter was selected under G418 pressure. In a 25cm(2) cell culture flask (5 ml culture medium), the cumulative protein yield of recombinant PoIFN-β reached 2.3×10(6) IU/ml. This cells clone maintained stable expression for at least 20 generations even in the absence of G418 selection pressure. The expressed recombinant PoIFN-β could induce the expression of porcine Mx protein in PK15 cells, and activate th ...[more]
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