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Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells.

ABSTRACT: A novel betacoronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which caused a large respiratory outbreak in Wuhan, China in December 2019, is currently spreading across many countries globally. Here, we show that a TMPRSS2-expressing VeroE6 cell line is highly susceptible to SARS-CoV-2 infection, making it useful for isolating and propagating SARS-CoV-2. Our results reveal that, in common with SARS- and Middle East respiratory syndrome-CoV, SARS-CoV-2 infection is enhanced by TMPRSS2.

SUBMITTER: Matsuyama S

PROVIDER: S-EPMC7132130 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells.

Matsuyama Shutoku S Nao Naganori N Shirato Kazuya K Kawase Miyuki M Saito Shinji S Takayama Ikuyo I Nagata Noriyo N Sekizuka Tsuyoshi T Katoh Hiroshi H Kato Fumihiro F Sakata Masafumi M Tahara Maino M Kutsuna Satoshi S Ohmagari Norio N Kuroda Makoto M Suzuki Tadaki T Kageyama Tsutomu T Takeda Makoto M

Proceedings of the National Academy of Sciences of the United States of America 20200312 13

A novel betacoronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which caused a large respiratory outbreak in Wuhan, China in December 2019, is currently spreading across many countries globally. Here, we show that a TMPRSS2-expressing VeroE6 cell line is highly susceptible to SARS-CoV-2 infection, making it useful for isolating and propagating SARS-CoV-2. Our results reveal that, in common with SARS- and Middle East respiratory syndrome-CoV, SARS-CoV-2 infection is enhance ...[more]

PMID: 32165541

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