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Methods for independently manipulating palatability and color in small insect prey.

ABSTRACT: Understanding how the psychology of predators shapes the defenses of colorful aposematic prey has been a rich area of inquiry, with emphasis on hypothesis-driven experiments that independently manipulate color and palatability in prey to examine predator responses. Most of these studies focus on avian predators, despite calls to consider more taxonomically diverse predators. This taxonomic bias leaves gaps in our knowledge about the generalizability of current theory. Here we have adapted tools that have been successfully used with bird predators and scaled them down and tested them with smaller predators (Habronattus jumping spiders) and small insect prey (termites, milkweed bug nymphs, pinhead crickets, fruit flies). Specifically, we test (1) the application of denatonium benzoate (DB) to the surface of live termites, crickets, and fruit flies, and (2) the effectiveness of manipulating the palatability of milkweed bug nymphs through diet. We also test the effectiveness of combining these palatability manipulations with various color manipulations. Across several experiments, we confirm that our palatability manipulations are not detectable to the spiders before they attack (i.e., they do not produce aversive odors that spiders avoid), and show that unpalatable prey are indeed quickly rejected and spiders do not habituate to the taste with experience. We also investigate limitations of these techniques by assessing possible unintended effects on prey behavior and the risk of contact contamination when using DB-treated prey in experiments. While similar tools have been used to manipulate color and palatability with avian predators and relatively large insect prey, we show how these techniques can be effectively adapted for use with small invertebrate predators and prey.

SUBMITTER: Winsor AM

PROVIDER: S-EPMC7138310 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Methods for independently manipulating palatability and color in small insect prey.

Winsor Alex M AM Ihle Malika M Taylor Lisa A LA

PloS one 20200407 4

Understanding how the psychology of predators shapes the defenses of colorful aposematic prey has been a rich area of inquiry, with emphasis on hypothesis-driven experiments that independently manipulate color and palatability in prey to examine predator responses. Most of these studies focus on avian predators, despite calls to consider more taxonomically diverse predators. This taxonomic bias leaves gaps in our knowledge about the generalizability of current theory. Here we have adapted tools ...[more]

PMID: 32255810

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Project description:Intraguild predation (IGP) is common in the freshwater insect communities, involving a top predator, intraguild prey (IG prey) and a shared prey. Influence of the habitat complexity on the prey-predator interactions is well established through several studies. In the present instance, the IGP involving the heteropteran predators and the dipteran prey were assessed in the background of the habitat complexity. The three predators Diplonychus rusticus, Ranatra filiformis, and Laccotrephes griseus, one intraguild prey Anisops bouvieri and two dipteran prey Culex quinquefasciatus and Chironomus sp. were used in different relative density against the complex habitat conditions to deduce the impact on the mortality on the prey. In comparison to the open conditions, the presence of the macrophytes and pebbles reduced the mortality of the shared prey under intraguild system as well as single predator system. The mortality of the shared prey was however dependent on the density of the predator and prey. Considering the shared prey mortality, predation on mosquito larvae was always higher in single predator system than chironomid larvae irrespective of identity and density of predators. However, for both the shared prey, complexity of habitat reduced the prey vulnerability in comparison to the simple habitat condition. Higher observed prey consumption depicts the higher risk to predation of shared prey, though the values varied with habitat conditions. Mortality of IG prey (A. bouvieri) in IGP system followed the opposite trend of the shared prey. The lower mortality in simple habitat and higher mortality in complex habitat conditions was observed for the IG prey, irrespective of shared prey and predator density. In IGP system, the shared prey mortality was influenced by the habitat conditions, with more complex habitat reducing the vulnerability of the shared prey and increased mortality of the IG prey. This implies that the regulation of the mosquitoes, in the IGP system will be impeded by the habitat conditions, with the heteropteran predators as the top predator.

Dataset Information

Methods for independently manipulating palatability and color in small insect prey.

Publications

Methods for independently manipulating palatability and color in small insect prey.

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