Ontology highlight
ABSTRACT:
SUBMITTER: Zhou XF
PROVIDER: S-EPMC7138890 | biostudies-literature | 2020 Apr
REPOSITORIES: biostudies-literature
Zhou Xiao-Feng XF Zhang Chen-Lu CL Gao Xue-Ping XP Wang Wei-Long WL He Zheng-Fen ZF Jiang Feng-Ying FY Pang Yi-Lin YL Li Jiang-Hui JH Ren Xiao-Jun XJ Zhou Huai-Bin HB Tan Guo-Qiang GQ Lyu Jian-Xin JX Wang Wu W
AMB Express 20200407 1
The development of novel methods for highly efficient protein purification remains a research focus in the biotechnology field because conventional purification approaches, including affinity purification, gel filtration, and ion-exchange chromatography, require complex manipulation steps and are costly. Here, we describe a simple and rapid protein purification strategy in which the SUMO tag and Ulp1 protease are surface-displayed separately on Escherichia coli cells. After protein induction, th ...[more]