Dataset Information

Molecular Characterization of PGC-1? (PPAR Gamma Coactivator 1?) and its Roles in Mitochondrial Biogenesis in Blunt Snout Bream (Megalobrama amblycephala).

ABSTRACT: This study aimed at achieving the molecular characterization of peroxisome proliferator-activated receptor-gamma coactivator 1? (PGC-1?) and exploring its modulatory roles in mitochondria biogenesis in blunt snout bream (Megalobrama amblycephala). A full-length cDNA of PGC-1? was cloned from liver which covered 3110 bp encoding 859 amino acids. The conserved motifs of PGC-1? family proteins were gained by MEME software, and the phylogenetic analyses showed motif loss and rearrangement of PGC-1? in fish. The function of PGC-1? was evaluated through overexpression and knockdown of PGC-1? in primary hepatocytes of blunt snout bream. We observed overexpression of PGC-1? along with enhanced mitochondrial transcription factor A (TFAM) expression and mtDNA copies in hepatocytes, and its knockdown led to slightly reduced NRF1 expression. However, knockdown of PGC-1? did not significantly influence TFAM expression or mtDNA copies. The alterations in mitochondria biogenesis were assessed following high-fat intake, and the results showed that it induces downregulation of PGC-1?. Furthermore, significant decreases in mitochondrial respiratory chain activities and mitochondria biogenesis were observed by high-fat intake. Our findings demonstrated that overexpression of PGC-1? induces the enhancement of TFAM expression and mtDNA amount but not NRF-1. Therefore, it could be concluded that PGC-1? is involved in mitochondrial biogenesis in blunt snout bream but not through PGC-1?/NRF-1 pathway.

SUBMITTER: Lu K

PROVIDER: S-EPMC7139572 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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Publications

Molecular Characterization of PGC-1β (PPAR Gamma Coactivator 1β) and its Roles in Mitochondrial Biogenesis in Blunt Snout Bream (Megalobrama amblycephala).

Lu Kangle K Policar Tomas T Song Xiaojun X Rahimnejad Samad S

International journal of molecular sciences 20200312 6

This study aimed at achieving the molecular characterization of peroxisome proliferator-activated receptor-gamma coactivator 1β (PGC-1β) and exploring its modulatory roles in mitochondria biogenesis in blunt snout bream (Megalobrama amblycephala). A full-length cDNA of PGC-1β was cloned from liver which covered 3110 bp encoding 859 amino acids. The conserved motifs of PGC-1β family proteins were gained by MEME software, and the phylogenetic analyses showed motif loss and rearrangem ...[more]

PMID: 32178369

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Project description:BackgroundBlunt snout bream (Megalobrama amblycephala) is an herbivorous freshwater fish species native to China and has been recognized as a main aquaculture species in the Chinese freshwater polyculture system with high economic value. Right now, only limited EST resources were available for M. amblycephala. Recent advances in large-scale RNA sequencing provide a fast, cost-effective, and reliable approach to generate large expression datasets for functional genomic analysis, which is especially suitable for non-model species with un-sequenced genomes.Methodology and principal findingsUsing 454 pyrosequencing, a total of 1,409,706 high quality reads (total length 577 Mbp) were generated from the normalized cDNA of pooled M. amblycephala individuals. These sequences were assembled into 26,802 contigs and 73,675 singletons. After BLAST searches against the NCBI non-redundant (NR) and UniProt databases with an arbitrary expectation value of E(-10), over 40,000 unigenes were functionally annotated and classified using the FunCat functional annotation scheme. A comparative genomics approach revealed a substantial proportion of genes expressed in M. amblycephala tanscriptome to be shared across the genomes of zebrafish, medaka, tetraodon, fugu, stickleback, human, mouse, and chicken, and identified a substantial number of potentially novel M. amblycephala genes. A total number of 4,952 SSRs were found and 116 polymorphic loci have been characterized. A significant number of SNPs (25,697) and indels (23,287) were identified based on specific filter criteria in the M. amblycephala.ConclusionsThis study is the first comprehensive transcriptome analysis for a fish species belonging to the genus Megalobrama. These large EST resources are expected to be valuable for the development of molecular markers, construction of gene-based linkage map, and large-scale expression analysis of M. amblycephala, as well as comparative genome analysis for the genus Megalobrama fish species. The identified SSR and SNP markers will greatly benefit its breeding program and whole genome association studies.

Dataset Information

Molecular Characterization of PGC-1? (PPAR Gamma Coactivator 1?) and its Roles in Mitochondrial Biogenesis in Blunt Snout Bream (Megalobrama amblycephala).

Publications

Molecular Characterization of PGC-1β (PPAR Gamma Coactivator 1β) and its Roles in Mitochondrial Biogenesis in Blunt Snout Bream (<i>Megalobrama amblycephala</i>).

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