Perturbing Endothelial Biomechanics via Connexin 43 Structural Disruption.
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ABSTRACT:
SUBMITTER: Islam MM
PROVIDER: S-EPMC7141411 | biostudies-literature | 2019 Oct
REPOSITORIES: biostudies-literature
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Perturbing Endothelial Biomechanics via Connexin 43 Structural Disruption.
Islam Md Mydul MM Steward Robert L RL
Journal of visualized experiments : JoVE 20191004 152
Endothelial cells have been established to generate intercellular stresses and tractions, but the role gap junctions play in endothelial intercellular stress and traction generation is currently unknown. Therefore, we present here a mechanics-based protocol to probe the influence of gap junction connexin 43 (Cx43) has on endothelial biomechanics by exposing confluent endothelial monolayers to a known Cx43 inhibitor 2,5-dihydroxychalcone (chalcone) and measuring the impact this inhibitor has on t ...[more]
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