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Comparison of Methods for Measuring Protein Concentration in Venom Samples.

ABSTRACT: Snake venom is an extremely interesting natural mixture of proteins and peptides, characterized by both high diversity and high pharmacological potential. Much attention has been paid to the study of venom composition of different species and also detailed analysis of the properties of individual components. Since proteins and peptides are the active ingredients in venom, rapidly developing proteomic techniques are used to analyze them. During such analyses, one of the routine operations is to measure the protein concentration in the sample. The aim of this study was to compare five methods used to measure protein content in venoms of two snake species: the Viperids representative, Agkistrodon contortrix, and the Elapids representative, Naja ashei. The study showed that for A. contortrix venom, the concentration of venom protein measured by four methods is very similar and only the NanoDrop method clearly stands out from the rest. However, in the case of N. ashei venom, each technique yields significantly different results. We hope that this report will help to draw attention to the problem of measuring protein concentration, especially in such a complex mixture as animal venoms.

SUBMITTER: Bocian A

PROVIDER: S-EPMC7142616 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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Comparison of Methods for Measuring Protein Concentration in Venom Samples.

Bocian Aleksandra A Sławek Sonja S Jaromin Marcin M Hus Konrad K KK Buczkowicz Justyna J Łysiak Dawid D Petrílla Vladimir V Petrillova Monika M Legáth Jaroslav J

Animals : an open access journal from MDPI 20200308 3

Snake venom is an extremely interesting natural mixture of proteins and peptides, characterized by both high diversity and high pharmacological potential. Much attention has been paid to the study of venom composition of different species and also detailed analysis of the properties of individual components. Since proteins and peptides are the active ingredients in venom, rapidly developing proteomic techniques are used to analyze them. During such analyses, one of the routine operations is to m ...[more]

PMID: 32182656

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Dataset Information

Comparison of Methods for Measuring Protein Concentration in Venom Samples.

Publications

Comparison of Methods for Measuring Protein Concentration in Venom Samples.

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