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Improved LbCas12a variants with altered PAM specificities further broaden the genome targeting range of Cas12a nucleases.


ABSTRACT: The widespread use of Cas12a (formerly Cpf1) nucleases for genome engineering is limited by their requirement for a rather long TTTV protospacer adjacent motif (PAM) sequence. Here we have aimed to loosen these PAM constraints and have generated new PAM mutant variants of the four Cas12a orthologs that are active in mammalian and plant cells, by combining the mutations of their corresponding RR and RVR variants with altered PAM specificities. LbCas12a-RVRR showing the highest activity was selected for an in-depth characterization of its PAM preferences in mammalian cells, using a plasmid-based assay. The consensus PAM sequence of LbCas12a-RVRR resembles a TNTN motif, but also includes TACV, TTCV CTCV and CCCV. The D156R mutation in improved LbCas12a (impLbCas12a) was found to further increase the activity of that variant in a PAM-dependent manner. Due to the overlapping but still different PAM preferences of impLbCas12a and the recently reported enAsCas12a variant, they complement each other to provide increased efficiency for genome editing and transcriptome modulating applications.

SUBMITTER: Toth E 

PROVIDER: S-EPMC7144938 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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Improved LbCas12a variants with altered PAM specificities further broaden the genome targeting range of Cas12a nucleases.

Tóth Eszter E   Varga Éva É   Kulcsár Péter István PI   Kocsis-Jutka Virág V   Krausz Sarah Laura SL   Nyeste Antal A   Welker Zsombor Z   Huszár Krisztina K   Ligeti Zoltán Z   Tálas András A   Welker Ervin E  

Nucleic acids research 20200401 7


The widespread use of Cas12a (formerly Cpf1) nucleases for genome engineering is limited by their requirement for a rather long TTTV protospacer adjacent motif (PAM) sequence. Here we have aimed to loosen these PAM constraints and have generated new PAM mutant variants of the four Cas12a orthologs that are active in mammalian and plant cells, by combining the mutations of their corresponding RR and RVR variants with altered PAM specificities. LbCas12a-RVRR showing the highest activity was select  ...[more]

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