Ontology highlight
ABSTRACT:
SUBMITTER: Toth E
PROVIDER: S-EPMC7144938 | biostudies-literature | 2020 Apr
REPOSITORIES: biostudies-literature
Tóth Eszter E Varga Éva É Kulcsár Péter István PI Kocsis-Jutka Virág V Krausz Sarah Laura SL Nyeste Antal A Welker Zsombor Z Huszár Krisztina K Ligeti Zoltán Z Tálas András A Welker Ervin E
Nucleic acids research 20200401 7
The widespread use of Cas12a (formerly Cpf1) nucleases for genome engineering is limited by their requirement for a rather long TTTV protospacer adjacent motif (PAM) sequence. Here we have aimed to loosen these PAM constraints and have generated new PAM mutant variants of the four Cas12a orthologs that are active in mammalian and plant cells, by combining the mutations of their corresponding RR and RVR variants with altered PAM specificities. LbCas12a-RVRR showing the highest activity was select ...[more]