Ontology highlight
ABSTRACT:
SUBMITTER: Chen Y
PROVIDER: S-EPMC7145522 | biostudies-literature | 2019 Sep
REPOSITORIES: biostudies-literature
Chen Yu Y Zhang Haiping H Xu Zhu Z Tang Huanyin H Geng Anke A Cai Bailian B Su Tao T Shi Jiejun J Jiang Cizhong C Tian Xiao X Seluanov Andrei A Huang Jun J Wan Xiaoping X Jiang Ying Y Gorbunova Vera V Mao Zhiyong Z
Nucleic acids research 20190901 16
Creating access to DNA double-strand break (DSB) sites in the chromatin context is an essential step during the repair process, but much remains to be determined about its regulatory mechanisms. Here, using a novel reporter cassette for simultaneous detection of homologous recombination (HR) and nonhomologous end joining (NHEJ) at the same chromosomal site, we report that the efficiency of HR but not NHEJ negatively correlates with nucleosome density. We demonstrate that PARP1 is required for HR ...[more]