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FBXO47 regulates telomere-inner nuclear envelope integration by stabilizing TRF2 during meiosis.

ABSTRACT: During meiosis, telomere attachment to the inner nuclear envelope is required for proper pairing of homologous chromosomes and recombination. Here, we identified F-box protein 47 (FBXO47) as a regulator of the telomeric shelterin complex that is specifically expressed during meiotic prophase I. Knockout of Fbxo47 in mice leads to infertility in males. We found that the Fbxo47 deficient spermatocytes are unable to form a complete synaptonemal complex. FBXO47 interacts with TRF1/2, and the disruption of Fbxo47 destabilizes TRF2, leading to unstable telomere attachment and slow traversing through the bouquet stage. Our findings uncover a novel mechanism of FBXO47 in telomeric shelterin subunit stabilization during meiosis.

SUBMITTER: Hua R

PROVIDER: S-EPMC7145685 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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FBXO47 regulates telomere-inner nuclear envelope integration by stabilizing TRF2 during meiosis.

Hua Rong R Wei Huafang H Liu Chao C Zhang Yue Y Liu Siyu S Guo Yueshuai Y Cui Yiqiang Y Zhang Xin X Guo Xuejiang X Li Wei W Liu Mingxi M

Nucleic acids research 20191201 22

During meiosis, telomere attachment to the inner nuclear envelope is required for proper pairing of homologous chromosomes and recombination. Here, we identified F-box protein 47 (FBXO47) as a regulator of the telomeric shelterin complex that is specifically expressed during meiotic prophase I. Knockout of Fbxo47 in mice leads to infertility in males. We found that the Fbxo47 deficient spermatocytes are unable to form a complete synaptonemal complex. FBXO47 interacts with TRF1/2, and the disrupt ...[more]

PMID: 31724724

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