Dataset Information

Wheel Alignment of a Suspension Module Unit Using a Laser Module.

ABSTRACT: Vehicle wheel alignment inspection is generally carried out using a computer vision-based system. Due to its inspection mechanism using four wheel centers, the computer vision-based system cannot be applied to the wheel alignment inspection of suspension module units. However, when a vehicle suspension module is being developed, there is no complete car ready for wheel alignment inspection even though it is a very important procedure for suspension property tests. This study proposes a novel and efficient way to inspect vehicle wheel alignment for suspension modules. Two laser modules and several mechanical jigs were employed for wheel alignment inspection, allowing the toe and camber angles of the suspension module to be measured. For accurate wheel alignment results, calibration of the laser modules was performed prior to the inspection. This calibration procedure adjusts the yaw and pitch angles of the laser module so that they can be orthogonal to the mounting jig. For the calibration, a novel method of using laser straightness was adopted and, consequently, 0.02 degrees of orthogonality was achieved. The wheel alignment inspection results were determined then verified using a vision system with two cameras. In order to use this vision system, two cameras were used and a new method of modifying the measurement mechanism was developed. According to the verification results, the proposed wheel alignment inspection provided very high measurement accuracy. The wheel alignment inspection mechanism proposed in this study can not only give very reliable results but also provide a cost-efficient method of inspecting the wheel alignment of suspension modules to automakers.

SUBMITTER: Kim SH

PROVIDER: S-EPMC7146572 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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Wheel Alignment of a Suspension Module Unit Using a Laser Module.

Kim Seong Han SH Lee Kang In KI

Sensors (Basel, Switzerland) 20200316 6

Vehicle wheel alignment inspection is generally carried out using a computer vision-based system. Due to its inspection mechanism using four wheel centers, the computer vision-based system cannot be applied to the wheel alignment inspection of suspension module units. However, when a vehicle suspension module is being developed, there is no complete car ready for wheel alignment inspection even though it is a very important procedure for suspension property tests. This study proposes a novel and ...[more]

PMID: 32188056

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Project description:BackgroundMesenchymal stromal cells (MSCs) are increasingly employed in regenerative medicine approaches for their immunomodulatory and anti-inflammatory properties, which are encoded in their secretome including extracellular vesicles (EVs). The Hoffa fat pad (HFP) located infrapatellarly harbours MSCs that could assist in tissue homeostasis in osteoarthritic joints. Intraarticular injection therapies based on blood products could modulate the populations of released HFP-MSC-EVs in a quantitative manner.MethodsTo obtain amounts of HFP-MSC-derived EVs that allow pre-clinical evaluation, suitable EV production systems need to be developed. This work investigates the release of EVs from primary HFP-MSCs cultivated in a 3D environment using microcarrier suspension culture in a vertical wheel bioreactor in comparison to conventional 2D culture. To simulate an intraarticular blood product therapy, cultures were treated with citrate-anticoagulated platelet-rich plasma (CPRP) or hyperacute serum (hypACT) before EV collection. HFP-MSC-EVs are enriched via ultrafiltration and characterised via Western Blot, nanoparticle tracking analysis in scatter as well as fluorescence mode. EV potency was determined via RT-qPCR analysing the expression of type II and X collagen (COL2 and COL10), as well as inducible nitric oxide synthase (iNOS) in primary OA chondrocytes.ResultsBlood product supplementation elevated HFP-MSC metabolic activity as determined via XTT assay over the course of 14 days. 3D culture resulted in a roughly 100-fold EV yield compared to 2D culture and elevated number of EVs released per cell. Total protein content correlated with the EV concentration. While typical EV marker proteins such as CD9, CD63 or Alix were detected in total protein extracts, CD9 and CD73 colocalised on individual EVs highlighting their cell origin. The type of blood product treatment did not affect the size or concentration of EVs obtained from HFP-MSCs. Assessing potency of 3D culture EVs in comparison to 2D EVs revealed superior biological activity with regard to inhibition of inflammation, inhibition of chondrocyte hypertrophy and induction of cartilage-specific ECM production.ConclusionsHFP-MSCs proliferate in presence of human blood products indicating that animal serum in culture media can be avoided in the future. The culture of HFP-MSCs in the employed bioreactor was successfully used to generate quantities of EVs that could allow evaluation of HFP-MSC-EV-mediated effects in pre-clinical settings. In addition, EV potency of 3D EVs is superior to EVs obtained in conventional 2D culture flasks.

Dataset Information

Wheel Alignment of a Suspension Module Unit Using a Laser Module.

Publications

Wheel Alignment of a Suspension Module Unit Using a Laser Module.

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