Unknown

Dataset Information

0

MiR-146a Ameliorates Hemoglobin-Induced Microglial Inflammatory Response via TLR4/IRAK1/TRAF6 Associated Pathways.


ABSTRACT: Microglial activation and sustained inflammation in the brain can lead to neuronal damage. Hence, limiting microglial activation and brain inflammation is a good therapeutic strategy for inflammatory-associated central nervous disease. MiR-146a is a promising therapeutic microRNA, since it can negatively regulate the inflammatory response. We thus investigated the expression changes of miR-146a after experimental induction of a subarachnoid hemorrhage (SAH) in vivo and in vitro, and we assessed the anti-inflammatory effects of miR-146a in microglial cells in vitro. Primary microglial cells were preincubated with miR-146a before hemoglobin (Hb) treatment. The results indicated that miR-146a decreased gene expression of Hb-induced pro-inflammatory cytokines (TNF-? and IL-1?) and phenotype-related genes (iNOS and CD86) through IRAK1/TRAF6/NF-?B or MAPK signaling pathways, suggesting its pro-resolution activity in microglia. However, contrary to the LPS-induced microglia or macrophage activation model, we did not observe an elevation in miR-146a after activation. Overall, our findings demonstrated that miR-146a was involved in the regulation of brain inflammation and could be considered a novel therapeutic agent for treating brain inflammation.

SUBMITTER: Liu GJ 

PROVIDER: S-EPMC7147172 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

altmetric image

Publications

MiR-146a Ameliorates Hemoglobin-Induced Microglial Inflammatory Response via TLR4/IRAK1/TRAF6 Associated Pathways.

Liu Guang-Jie GJ   Zhang Qing-Rong QR   Gao Xuan X   Wang Han H   Tao Tao T   Gao Yong-Yue YY   Zhou Yan Y   Chen Xiang-Xin XX   Li Wei W   Hang Chun-Hua CH  

Frontiers in neuroscience 20200403


Microglial activation and sustained inflammation in the brain can lead to neuronal damage. Hence, limiting microglial activation and brain inflammation is a good therapeutic strategy for inflammatory-associated central nervous disease. MiR-146a is a promising therapeutic microRNA, since it can negatively regulate the inflammatory response. We thus investigated the expression changes of miR-146a after experimental induction of a subarachnoid hemorrhage (SAH) <i>in vivo</i> and <i>in vitro</i>, an  ...[more]

Similar Datasets

| S-EPMC4079695 | biostudies-literature
| S-EPMC3841223 | biostudies-literature
| S-EPMC9929824 | biostudies-literature
| S-EPMC6913124 | biostudies-literature
| S-EPMC5994250 | biostudies-literature
| S-EPMC7086938 | biostudies-literature
| S-EPMC4317016 | biostudies-literature
| S-EPMC5455804 | biostudies-literature
| S-EPMC8506750 | biostudies-literature
| S-EPMC9281091 | biostudies-literature