Dataset Information

Effects of Skin Lightening Cream Agents - Hydroquinone and Kojic Acid, on the Skin of Adult Female Experimental Rats.

ABSTRACT: Introduction:Skin bleaching is the act of using steroid- and chemical-containing products to lighten the skin. Hydroquinone and kojic acid are often used in skin bleaching creams. Hydroquinone was suspected to be harmful. This study investigated the effects of kojic acid and hydroquinone on the skin of adult female Wistar rats and the potential use of aloe vera for amelioration. Materials and Methods:Eighty [n=80] adult female Wistar rats with an average weight of 120 g were randomly divided into eight groups, marked A, B, C, D, E, F, G, and H. Group A animals served as the control group; group B was treated with 2% hydroquinone, group C was treated with 2% kojic acid, group D was treated with 4% hydroquinone, group E was treated with 4% kojic acid, group F was treated with 2% hydroquinone and 2% kojic acid, group G was treated with 4% hydroquinone and 4% kojic acid, and group H was treated with 4% hydroquinone, 4% kojic acid, and aloe vera. The preparations were applied to the tail skin and treatment lasted 28 days. Skin samples were excised and processed using H&E, Masson's trichrome, and p65 immunohistochemical assays on tissue sections. Results:Hydroquinone caused structural disruptions of the stratum corneum of the epidermis and the overlying keratin. p65 was also prominently expressed in the treated groups. Hydroquinone reduced skin thickness and caused epidermis disruption. Discussion:The prominent expression of p65 in the sections indicated deleterious effects of hydroquinone. Kojic acid was not found to have deleterious effects. Aloe vera prevented extensive disruption of stratum corneum by hydroquinone. The use of hydroquinone in skin lightening creams might raise health concerns. Aloe vera could be protective against hydroquinone.

SUBMITTER: Owolabi JO

PROVIDER: S-EPMC7147621 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Effects of Skin Lightening Cream Agents - Hydroquinone and Kojic Acid, on the Skin of Adult Female Experimental Rats.

Owolabi Joshua Oladele JO Fabiyi Oluseyi Sunday OS Adelakin Lola Adeola LA Ekwerike Miriammillicent Chinenyenwa MC

Clinical, cosmetic and investigational dermatology 20200406

<h4>Introduction</h4>Skin bleaching is the act of using steroid- and chemical-containing products to lighten the skin. Hydroquinone and kojic acid are often used in skin bleaching creams. Hydroquinone was suspected to be harmful. This study investigated the effects of kojic acid and hydroquinone on the skin of adult female Wistar rats and the potential use of aloe vera for amelioration.<h4>Materials and methods</h4>Eighty [n=80] adult female Wistar rats with an average weight of 120 g were rando ...[more]

PMID: 32308462

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Project description:BackgroundSkin-lightening (SL) products are common, especially in Africa. Adverse effects from these products represent a public health concern. Use of these products in Somaliland is unknown.ObjectiveThis study aimed to determine the prevalence of use of SL products among female health science students, beliefs about these products and practices, and adverse effects experienced.MethodsThis was a cross-sectional survey of a convenience sample of female health science students at Amoud University in Borama, Somaliland.ResultsOf the 400 students who were invited to participate, 265 completed the survey (response rate: 66%). Mean participant age was 21.1 years (standard deviation: 2.0 years). The majority of students were single (91.2%) with a Fitzpatrick skin type of 3 or darker (94.2%). Past or present use of SL products was reported by 25.6% of participants, and 52.2% admitted to current use. Compared with non-users, more SL product users agreed that lighter skin color gives a woman more confidence, helps a woman have better job opportunities, and increases chances of getting married. They also agreed that advertisements on television for SL products influence a women's preference for a lighter skin tone (p < .05). More than 60% of participants were unsure what active ingredients were in their SL products. Only 9% denied any undesirable adverse effects, and the remainder reported an array of local and systemic adverse effects. The vast majority realize that SL products may cause undesirable local (92%) and systemic (89%) adverse effects.LimitationsGeneralizability is limited because a nonrandomized convenience sample from one university was studied. Response bias also may have skewed results.ConclusionUse of SL products among female health science students in Somaliland is common, and causes cutaneous and systemic adverse reactions. Use appears influenced by beliefs about the benefits of lighter skin color. Education is needed on the proper use of these products, how to avoid harmful products, and how to prevent complications.

Project description:BackgroundStudies on the impact of the skin microbiota on human health have been gaining more attention. Bacteria are associated with various diseases, although certain strains of bacteria, which are known as probiotics, are considered beneficial. Mixtures of several bacteria (bacterial cocktail) isolated from targeted organs have shown promising modulatory activities for use in skin therapeutics. The objectives of this study were to determine and identify the microbial communities on the skin that can potentially be used as probiotics, as determined by bacterial isolation and cultivation, followed by next-generation sequencing (NGS).ResultsSamples were collected by swabbing on forehead and cheek skin. Genomic DNA from bacterial swab samples were directly extracted to be further processed into NGS. Cultivation of skin bacteria was carried out in subsequent medium. Thus, around twenty bacterial isolates with different characteristics were selected and identified by both culture-based method and 16sRNA sequencing. We found that Actinobacteria and Firmicutes are the most abundant phylum present on the skin as presented by NGS data, which constitute to 67% and 28.59% of the whole bacterial population, consecutively. However, Staphylococcus hominis, Staphylococcus warneri, and Micrococcus luteus (AN MK968325.1; AN MK968315.1; and MK968318.1 respectively) were able to be obtained in the samples of cultivable, and could be potentially developed as probiotics in skin microbiome therapeutic as well as for postbiotic formulation.ConclusionSkin microbiome is considered to provide several probiotics for skin therapeutic. However, some opportunistic pathogens were discovered in this study population. Thus, the promising formula of bacterial cocktail for skin microbiome therapeutic must be thoroughly elucidated to avoid unwanted species. Our study is the first human skin microbiome profile of Indonesia resulted from a Next Generation Sequencing as an effort to show a representative of tropical country profile.

Dataset Information

Effects of Skin Lightening Cream Agents - Hydroquinone and Kojic Acid, on the Skin of Adult Female Experimental Rats.

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Effects of Skin Lightening Cream Agents - Hydroquinone and Kojic Acid, on the Skin of Adult Female Experimental Rats.

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