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ABSTRACT: Context
Inflammation and insulin resistance are often present in polycystic ovary syndrome (PCOS).Objective
We determined the effect of saturated fat ingestion on mononuclear cell (MNC) nuclear factor-?B (NF?B) activation; NF?B, inhibitory-?B? (I?B?), and tumor necrosis factor-? (TNF?) gene expression; and circulating C-reactive protein (CRP) in women with PCOS.Design
Cross-sectional study.Setting
Academic medical center.Patients
Twenty reproductive-age women with PCOS (10 lean, 10 with obesity) and 20 ovulatory controls (10 lean, 10 with obesity).Main outcome measures
Activated NF?B, NF?B heterodimer subunits, I?B? and TNF? messenger ribonucleic acid content and NF?B p65 and I?B? protein content were quantified in mononuclear cells (MNC), and CRP was measured in plasma from blood drawn fasting and 2, 3, and 5 h after saturated fat ingestion. Insulin sensitivity was derived from oral glucose tolerance testing (ISOGTT). Androgen secretion was assessed from blood drawn fasting and 24, 48, and 72 h after human chorionic gonadotropin (HCG) administration.Results
In response to saturated fat ingestion, women with PCOS regardless of weight class exhibited lipid-induced increases in activated NF?B, NF?B, and TNF? gene expression and plasma CRP and decreases in I?B? protein compared with lean control subjects. Both PCOS groups exhibited lower ISOGTT and greater HCG-stimulated androgen secretion compared with control subjects. Lipid-stimulated NF?B activation was negatively correlated with ISOGTT, and positively correlated with HCG-stimulated androgen secretion.Conclusion
In PCOS, increases in NF?B activation and circulating CRP and decreases in I?B? protein following saturated fat ingestion are independent of obesity. Circulating MNC and excess adipose tissue are separate and distinct contributors to inflammation in this disorder.
SUBMITTER: Gonzalez F
PROVIDER: S-EPMC7150616 | biostudies-literature | 2020 Jun
REPOSITORIES: biostudies-literature
González Frank F Considine Robert V RV Abdelhadi Ola A OA Acton Anthony J AJ
The Journal of clinical endocrinology and metabolism 20200601 6
<h4>Context</h4>Inflammation and insulin resistance are often present in polycystic ovary syndrome (PCOS).<h4>Objective</h4>We determined the effect of saturated fat ingestion on mononuclear cell (MNC) nuclear factor-κB (NFκB) activation; NFκB, inhibitory-κBα (IκBα), and tumor necrosis factor-α (TNFα) gene expression; and circulating C-reactive protein (CRP) in women with PCOS.<h4>Design</h4>Cross-sectional study.<h4>Setting</h4>Academic medical center.<h4>Patients</h4>Twenty reproductive-age wo ...[more]