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Surface Chemistry-Dependent Evolution of the Nanomaterial Corona on TiO2 Nanomaterials Following Uptake and Sub-Cellular Localization.


ABSTRACT: Nanomaterial (NM) surface chemistry has an established and significant effect on interactions at the nano-bio interface, with important toxicological consequences for manufactured NMs, as well as potent effects on the pharmacokinetics and efficacy of nano-therapies. In this work, the effects of different surface modifications (PVP, Dispex AA4040, and Pluronic F127) on the uptake, cellular distribution, and degradation of titanium dioxide NMs (TiO2 NMs, ~10 nm core size) are assessed and correlated with the localization of fluorescently-labeled serum proteins forming their coronas. Imaging approaches with an increasing spatial resolution, including automated high throughput live cell imaging, correlative confocal fluorescence and reflectance microscopy, and dSTORM super-resolution microscopy, are used to explore the cellular fate of these NMs and their associated serum proteins. Uncoated TiO2 NMs demonstrate a rapid loss of corona proteins, while surface coating results in the retention of the corona signal after internalization for at least 24 h (varying with coating composition). Imaging with two-color super-resolution dSTORM revealed that the apparent TiO2 NM single agglomerates observed in diffraction-limited confocal microscopy are actually adjacent smaller agglomerates, and provides novel insights into the spatial arrangement of the initial and exchanged coronas adsorbed at the NM surfaces.

SUBMITTER: Khan AO 

PROVIDER: S-EPMC7152854 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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Surface Chemistry-Dependent Evolution of the Nanomaterial Corona on TiO<sub>2</sub> Nanomaterials Following Uptake and Sub-Cellular Localization.

Khan Abdullah O AO   Di Maio Alessandro A   Guggenheim Emily J EJ   Chetwynd Andrew J AJ   Pencross Dan D   Tang Selina S   Belinga-Desaunay Marie-France A MA   Thomas Steven G SG   Rappoport Joshua Z JZ   Lynch Iseult I  

Nanomaterials (Basel, Switzerland) 20200225 3


Nanomaterial (NM) surface chemistry has an established and significant effect on interactions at the nano-bio interface, with important toxicological consequences for manufactured NMs, as well as potent effects on the pharmacokinetics and efficacy of nano-therapies. In this work, the effects of different surface modifications (PVP, Dispex AA4040, and Pluronic F127) on the uptake, cellular distribution, and degradation of titanium dioxide NMs (TiO<sub>2</sub> NMs, ~10 nm core size) are assessed a  ...[more]

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