Dataset Information

Tetraploid Induction by Colchicine Treatment and Crossing with a Diploid Reveals Less-Seeded Fruit Production in Pointed Gourd (Trichosanthes dioica Roxb.).

ABSTRACT: Pointed gourd (Trichosanthes dioica Roxb.) (2n = 2x = 22) is a dioecious cucurbit vegetable and green fruit that is edible after cooking. Consumers prefer to consume seedless or less-seeded fruit because seeds are unpalatable due to their hard coats. Therefore, the cross compatibility between the diploid and induced tetraploid will be helpful for seedless or less-seeded fruit production. Thus, the present study was conducted using mature seeds that were immersed in 0.05%, 0.1%, and 0.5% colchicine for 24, 48, and 72 h to induce tetraploids. These tetraploids were used as parents (male or female) in the inter-ploidy and intra-ploidy crosses. A flow cytometric analysis confirmed the induction of three tetraploids at 0.5% colchicine for 48 and 72 h soaking periods. Among these, two (2) females and one (1) male were differentiated after flower initiation. Crossing between the tetraploid's maternal and diploid paternal parent (4x × 2x), which were revealed to be compatible, resulted in a similar fruit set rate and shape as those of the diploid. In addition, a seed number of 4x × 2x produced fruits that were drastically reduced to 1.8 seeds per fruit, whereas the natural diploid fruits had 26.4 seeds per fruit. These findings suggest that colchicine-induced tetraploid females are important genetic resources for less-seeded fruit production. The genetic stability of tetraploid clones can easily and effectively be maintained by vine cutting for advanced uses.

SUBMITTER: Hassan J

PROVIDER: S-EPMC7154917 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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Publications

Tetraploid Induction by Colchicine Treatment and Crossing with a Diploid Reveals Less-Seeded Fruit Production in Pointed Gourd (<i>Trichosanthes dioica</i> Roxb.).

Hassan Jahidul J Miyajima Ikuo I Ozaki Yukio Y Mizunoe Yuki Y Sakai Kaori K Zaland Wasimullah W

Plants (Basel, Switzerland) 20200317 3

Pointed gourd (<i>Trichosanthes dioica</i> Roxb.) (2n = 2x = 22) is a dioecious cucurbit vegetable and green fruit that is edible after cooking. Consumers prefer to consume seedless or less-seeded fruit because seeds are unpalatable due to their hard coats. Therefore, the cross compatibility between the diploid and induced tetraploid will be helpful for seedless or less-seeded fruit production. Thus, the present study was conducted using mature seeds that were immersed in 0.05%, 0.1%, and 0.5% c ...[more]

PMID: 32192090

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Project description:BackgroundSpine gourd (Momordica dioica Roxb. Willd) is one of the important cucurbitaceous crops grown across the world for vegetable and medicinal purposes. Diseases caused by the DNA viruses are becoming the limiting factors for the production of spine gourd reducing its potential yield. For the commercial cultivation of the spine gourd, propagation material used by most of the growers is tuberous roots and stem cuttings, which in turn results in an increased occurrence of the mosaic disease. There is a need for understanding the causal agent; through characterization of which will lead to the designing management strategies for the spine gourd mosaic disease control.ObjectivesCharacterization of a begomovirus and its satellites associated with mosaic disease on spine gourd.Materials and methodsTotal DNA was extracted from spine gourd samples exhibiting symptoms typical to the begomoviruses infection (mosaic mottling, leaf curl) and was tested by PCR using begomovirus specific primers. Furthermore, the complete genome of begomo viruses (DNA A, DNA B, alpha satellite, and beta satellite) was amplified by rolling circle amplification (RCA) method.ResultsThe full-length sequences of DNA A, DNA B, alpha satellite, and beta satellite isolated from symptomatic spine gourd were determined. The full length genomes (DNA A and DNA B) of the Tomato leaf curl New Delhi Virus (ToLCNDV) infecting spine gourd were compared with the other begomovirus genomes available in the data base. The sequence analysis has revealed that DNA A and DNA B components of the begomovirus infecting spine gourd share 95.4-96.2 and 86.7-91.2% identical sequence (i.e., nucleotide (nt) identity) with that of ToLCNDV infecting potato and cucurbits in the Indian subcontinent isolates reported earlier (available in GenBank), respectively. Further, alpha satellite and beta satellite were also detected in the begomovirus infected spine gourd samples. The recombination analysis of the DNA A, DNA B, beta satellite, and alpha satellite of the begomovirus infecting spine gourd showed the associated begomovirus and satellite DNAs were driven from the different begomoviruses, leading to emergence as a new variant of the begomovirus infecting spine gourd.ConclusionsThe commercial cultivation of the spine gourd by most growers depends on the tuberous roots and stem cutting. The occurrence of begomovirus in spine gourd gives an alarming signal against utilization of such infected plant materials in the crop breeding and improvement programs. Using the clean virus-free vegetative propagation material is considered as one of the most important methods for controlling viral diseases. The study is highly useful for detection of the begomovirus infecting spine gourd in the detection of the virus infection in the clonally propagated planting material.

Dataset Information

Tetraploid Induction by Colchicine Treatment and Crossing with a Diploid Reveals Less-Seeded Fruit Production in Pointed Gourd (Trichosanthes dioica Roxb.).

Publications

Tetraploid Induction by Colchicine Treatment and Crossing with a Diploid Reveals Less-Seeded Fruit Production in Pointed Gourd (<i>Trichosanthes dioica</i> Roxb.).

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