Ontology highlight
ABSTRACT:
SUBMITTER: Fu Y
PROVIDER: S-EPMC7155207 | biostudies-literature | 2020 Apr
REPOSITORIES: biostudies-literature
Fu Yajuan Y Fang Yijun Y Lin Zhang Z Yang Lei L Zheng Liqun L Hu Hao H Yu Tingting T Huang Baoting B Chen Suxing S Wang Hanze H Xu Shan S Bao Wei W Chen Qi Q Sun Lijun L
iScience 20200331 4
DNA transfection is often the bottleneck of research and gene therapy practices. To explore the mechanism regulating transgene expression, we investigated the role of the cGAS-STING signaling pathway, which induces type-I interferons in response to DNA. We confirmed that deletion of cGAS enhances transgene expression at the protein level by ~2- to 3-fold. This enhancement is inversely correlated with the expression of interferons and interferon stimulated genes (ISGs), which suppress expression ...[more]