Dataset Information

Determining nitrogen isotopes discrimination under drought stress on enzymatic activities, nitrogen isotope abundance and water contents of Kentucky bluegrass.

ABSTRACT: Drought stress is the most pervasive threat to plant growth, which predominantly encumbers turf grass growth by causing alterations in plant functions. This study appraised the role of nitrogen isotopes in providing a theoretical basis for developing and improving Kentucky bluegrass cultivar performance under drought stress. Nitrogen isotopes labelled 15NH4Cl and K15NO3 were prepared to replace KNO3 in Hoagland's solution at concentrations of 15NH4+ and 15NO3 at 1.5, 15, and 30?mM; the solutions were imposed on stressed plants under glasshouse conditions. Nitrogenous nutrition reduced oxidative stress by elevating the enzymatic activities and proline contents of all three clonal ramet leaves, particularly under stress conditions. Apart from nitrogen content, nitrogen isotope abundance, relative water content and water potential within controls were enhanced in treated with 15NH4+ than in with 15NO3 in both the roots and leaves of Kentucky bluegrass. Nevertheless, an application of 15NH4Cl and K15NO3 at 30?mM had a positive influence to some extent on these attributes under drought stress. Overall, our results suggested that nitrogen isotopes contributed to drought tolerance in all three clonal ramets of Kentucky bluegrass by maintaining a better osmoprotectant and antioxidant defence system, which helped the plants eliminate reactive oxygen species.

SUBMITTER: Saud S

PROVIDER: S-EPMC7156527 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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Determining nitrogen isotopes discrimination under drought stress on enzymatic activities, nitrogen isotope abundance and water contents of Kentucky bluegrass.

Saud Shah S Fahad Shah S Cui Guowen G Yajun Chen C Anwar Sumera S Anwar Sumera S

Scientific reports 20200414 1

Drought stress is the most pervasive threat to plant growth, which predominantly encumbers turf grass growth by causing alterations in plant functions. This study appraised the role of nitrogen isotopes in providing a theoretical basis for developing and improving Kentucky bluegrass cultivar performance under drought stress. Nitrogen isotopes labelled 15NH4Cl and K15NO3 were prepared to replace KNO3 in Hoagland's solution at concentrations o ...[more]

PMID: 32286483

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Project description:Continuous monoculture of cool-season turfgrass causes soil degradation, and visual turf quality decline is a major concern in black soil regions of Northeast China. Turf mixtures can enhance turfgrass resistance to biotic and abiotic stresses and increase soil microbial diversity. Understanding mechanism by plant-soil interactions and changes of black soil microbial communities in turf mixture is beneficial to restoring the degradation of urbanized black soils and maintaining sustainable development of urban landscape ecology. In this study, based on the previous research of different sowing models, two schemes of turf monoculture and mixture were conducted in field plots during 2016-2018 in a black soil of Heilongjiang province of Northeast China. The mixture turf was established by mixing 50% Kentucky bluegrass "Midnight" (Poa pratensis L.) with 50% Red fescue "Frigg" (Festuca rubra L.); and the monoculture turf was established by sowing with pure Kentucky bluegrass. Turf performance, soil physiochemical properties, and microbial composition from rhizosphere were investigated. Soil microbial communities and abundance were analyzed by Illumina MiSeq sequencing and quantitative PCR methods. Results showed that turfgrass quality, turfgrass biomass, soil organic matter (SOM), urease, alkaline phosphatase, invertase, and catalase activities increased in PF mixture, but disease percentage and soil pH decreased. The microbial diversity was also significantly enhanced under turf mixture model. The microbial community compositions were significantly different between the two schemes. Turf mixtures obviously increased the abundances of Beauveria, Lysobacter, Chryseolinea, and Gemmatimonas spp., while remarkably reduced the abundances of Myrothecium and Epicoccum spp. Redundancy analysis showed that the compositions of bacteria and fungi were related to edaphic parameters, such as SOM, pH, and enzyme activities. Since the increasing of turf quality, biomass, and disease resistance were highly correlated with the changes of soil physiochemical parameters and microbial communities in turf mixture, which suggested that turf mixture with two species (i.e., Kentucky blue grass and Red fescue) changed soil microbial communities and enhanced visual turfgrass qualities through positive plant-soil interactions by soil biota.

Dataset Information

Determining nitrogen isotopes discrimination under drought stress on enzymatic activities, nitrogen isotope abundance and water contents of Kentucky bluegrass.

Publications

Determining nitrogen isotopes discrimination under drought stress on enzymatic activities, nitrogen isotope abundance and water contents of Kentucky bluegrass.

Similar Datasets

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