Ontology highlight
ABSTRACT:
SUBMITTER: Reixachs-Sole M
PROVIDER: S-EPMC7156646 | biostudies-literature | 2020 Apr
REPOSITORIES: biostudies-literature
Reixachs-Solé Marina M Ruiz-Orera Jorge J Albà M Mar MM Eyras Eduardo E
Nature communications 20200414 1
The differential production of transcript isoforms from gene loci is a key cellular mechanism. Yet, its impact in protein production remains an open question. Here, we describe ORQAS (ORF quantification pipeline for alternative splicing), a pipeline for the translation quantification of individual transcript isoforms using ribosome-protected mRNA fragments (ribosome profiling). We find evidence of translation for 40-50% of the expressed isoforms in human and mouse, with 53% of the expressed gene ...[more]