Dataset Information

Diversity of Diptera Species in Estonian Pig Farms.

ABSTRACT: In light of the African swine fever outbreaks in Estonian pig farms during the past few years, the question of the vector potential of Diptera in the pig farm environment has risen. However, the arthropod fauna of the pig farm environment is currently not well established. Hence, the aim of this study was to clarify the species diversity in pig farms. In total, 22 Diptera species or species groups were found in Estonian pig farms. There were altogether 186,701 individual arthropods collected, from which 96.6% (180,444) belonged to the order of true flies (Insecta: Diptera). The remaining 3.4% were from other insect orders, arachnids, or just damaged and unidentifiable specimens. The activity density and diversity of dipterans differed significantly between 12 sampled farms but not throughout the sampling period. The present study is amongst the few to provide a large-scale overview of pig-farm-associated Diptera in the temperate climate zone.

SUBMITTER: Tummeleht L

PROVIDER: S-EPMC7157211 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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Diversity of Diptera Species in Estonian Pig Farms.

Tummeleht Lea L Jürison Margret M Kurina Olavi O Kirik Heli H Jeremejeva Julia J Viltrop Arvo A

Veterinary sciences 20200123 1

In light of the African swine fever outbreaks in Estonian pig farms during the past few years, the question of the vector potential of Diptera in the pig farm environment has risen. However, the arthropod fauna of the pig farm environment is currently not well established. Hence, the aim of this study was to clarify the species diversity in pig farms. In total, 22 Diptera species or species groups were found in Estonian pig farms. There were altogether 186,701 individual arthropods collected, fr ...[more]

PMID: 31979423

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Project description:Porcine sapelovirus (PSV) is a ubiquitous virus in farmed pigs that is associated with SMEDI syndrome, polioencephalomyelitis, and diarrhea. However, there are few reports on the prevalence and molecular characterization of PSV in Fujian Province, Southern China. In this study, the prevalence of PSV and a poetical combinative strain PSV2020 were characterized using real-time PCR, sequencing, and bioinformatics analysis. As a result, an overall sample prevalence of 30.8% was detected in 260 fecal samples, and a farm prevalence of 76.7% was observed in 30 Fujian pig farms, from 2020 to 2022. Noteably, a high rate of PSV was found in sucking pigs. Bioinformatics analysis showed that the full-length genome of PSV2020 was 7550 bp, and the genetic evolution of its ORF region was closest to the G1 subgroup, which was isolated from Asia and America; the similarity of nucleotides and amino acids to other PSVs was 59.5~88.7% and 51.7~97.0%, respectively. However, VP1 genetic evolution analysis showed a distinct phylogenetic topology from the ORF region; PSV2020 VP1 was closer to the DIAPD5469-10 strain isolated from Italy than strains isolated from Asia and America, which comprise the G1 subgroup based on the ORF region. Amino acid discrepancy analysis illustrated that the PSV2020 VP1 gene inserted twelve additional nucleotides, corresponding to four additional amino acids (STAE) at positions 898-902 AAs. Moreover, a potential recombination signal was observed in the 2A coding region, near the 3' end of VP1, owing to recombination analysis. Additionally, 3D genetic evolutionary analysis showed that all reference strains demonstrated, to some degree, regional conservation. These results suggested that PSV was highly prevalent in Fujian pig farms, and PSV2020, a PSV-1 genotype strain, showed gene diversity and recombination in evolutionary progress. This study also laid a scientific foundation for the investigation of PSV epidemiology, molecular genetic characteristics, and vaccine development.

Dataset Information

Diversity of Diptera Species in Estonian Pig Farms.

Publications

Diversity of Diptera Species in Estonian Pig Farms.

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