Dataset Information

Lab-Made Electronic Nose for Fast Detection of Listeria monocytogenes and Bacillus cereus.

ABSTRACT: The aim of this study is to determine the performance of a lab-made electronic nose (e-nose) composed of an array of metal oxide semiconductor (MOS) gas sensors in the detection and differentiation of Listeria monocytogenes (L. monocytogenes) and Bacillus cereus (B. cereus) incubated in trypticsoy broth (TSB) media. Conventionally, the detection of L. monocytogenes and B. cereus is often performed by enzyme link immunosorbent assay (ELISA) and polymerase chain reaction (PCR). These techniques require trained operators and expert, expensive reagents and specific containment. In this study, three types of samples, namely, TSB media, L. monocytogenes (serotype 4b American Type Culture Collection (ATCC) 13792), and B. cereus (ATCC) 10876, were used for this experiment. Prior to measurement using the e-nose, each bacterium was inoculated in TSB at 1 × 103-104 CFU/mL, followed by incubation for 48 h. To evaluate the performance of the e-nose, the measured data were then analyzed with chemometric models, namely linear and quadratic discriminant analysis (LDA and QDA), and support vector machine (SVM). As a result, the e-nose coupled with SVM showeda high accuracy of 98% in discriminating between TSB media and L. monocytogenes, and between TSB media and B. cereus. It could be concluded that the lab-made e-nose is able to detect rapidly the presence of bacteria L. monocytogenes and B. cereus on TSB media. For the future, it could be used to identify the presence of L. monocytogenes or B. cereus contamination in the routine and fast assessment of food products in animal quarantine.

SUBMITTER: Astantri PF

PROVIDER: S-EPMC7158669 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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Lab-Made Electronic Nose for Fast Detection of Listeria monocytogenes and Bacillus cereus.

Astantri Prima Febri PF Prakoso Wredha Sandhi Ardha WSA Triyana Kuwat K Untari Tri T Airin Claude Mona CM Astuti Pudji P

Veterinary sciences 20200209 1

The aim of this study is to determine the performance of a lab-made electronic nose (e-nose) composed of an array of metal oxide semiconductor (MOS) gas sensors in the detection and differentiation of Listeria monocytogenes (L. monocytogenes) and Bacillus cereus (B. cereus) incubated in trypticsoy broth (TSB) media. Conventionally, the detection of L. monocytogenes and B. cereus is often performed by enzyme link immunosorbent assay (ELISA) and polymerase ...[more]

PMID: 32050503

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Project description:Listeria monocytogenes is a foodborne pathogen that causes high rates of hospitalization and mortality in people infected. Contamination of fresh, ready to eat produce by this pathogen is especially troubling because of the ability of this bacterium to grow on produce under refrigeration temperatures. In this study, we created a library of over 8,000 plant phyllosphere-associated bacteria and screened them for the ability to inhibit the growth of L. monocytogenes in an in vitro fluorescence-based assay. One isolate, later identified as Bacillus amyloliquefaciens ALB65, was able to inhibit the fluorescence of L. monocytogenes by >30-fold in vitro. B. amyloliquefaciens ALB65 was also able to grow, persist, and reduce the growth of L. monocytogenes by >1.5 log CFU on cantaloupe melon rinds inoculated with 5 × 103 CFU at 30°C and was able to completely inhibit its growth at temperatures below 8°C. DNA sequence analysis of the B. amyloliquefaciens ALB65 genome revealed six gene clusters that are predicted to encode genes for antibiotic production; however, no plant or human virulence factors were identified. These data suggest that B. amyloliquefaciens ALB65 is an effective and safe biological control agent for the reduction of L. monocytogenes growth on intact cantaloupe melons and possibly other types of produce.IMPORTANCEListeria monocytogenes is estimated by the Centers for Disease Control and Prevention and the U.S. Food and Drug Administration to cause disease in approximately 1,600 to 2,500 people in the United States every year. The largest known outbreak of listeriosis in the United States was associated with intact cantaloupe melons in 2011, resulting in 147 hospitalizations and 33 deaths. In this study, we demonstrated that Bacillus amyloliquefaciens ALB65 is an effective biological control agent for the reduction of L. monocytogenes growth on intact cantaloupe melons under both pre- and postharvest conditions. Furthermore, we demonstrated that B. amyloliquefaciens ALB65 can completely inhibit the growth of L. monocytogenes during cold storage (<8°C).

Dataset Information

Lab-Made Electronic Nose for Fast Detection of Listeria monocytogenes and Bacillus cereus.

Publications

Lab-Made Electronic Nose for Fast Detection of <i>Listeria monocytogenes</i> and <i>Bacillus cereus</i>.

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