Development of qPCR platform with probes for quantifying prevalent and biomedically relevant human gut microbial taxa.
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ABSTRACT:
SUBMITTER: Kurina I
PROVIDER: S-EPMC7158812 | biostudies-literature | 2020 Apr
REPOSITORIES: biostudies-literature
Publications
Development of qPCR platform with probes for quantifying prevalent and biomedically relevant human gut microbial taxa.
Kurina Irina I Popenko Anna A Klimenko Natalia N Koshechkin Stanislav S Chuprikova Liubov L Filipenko Maxim M Tyakht Alexander A Alexeev Dmitry D
Molecular and cellular probes 20200415
Nowadays the advent of innovative high-throughput sequencing allows obtaining high-quality microbiome profiling. However, PCR-based tests are still considered the "golden standard" for many clinical applications. Here, we designed a qPCR-based platform with fluorescent-labeled oligonucleotide probes for assessing human gut microbiome composition. The system allows conducting qualitative and semiquantitative analysis for 12 prokaryotic taxa that are prevalent in the human gut and associated with ...[more]
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