Dataset Information

Age-related transcriptional modules and TF-miRNA-mRNA interactions in neonatal and infant human thymus.

ABSTRACT: The human thymus suffers a transient neonatal involution, recovers and then starts a process of decline between the 1st and 2nd years of life. Age-related morphological changes in thymus were extensively investigated, but the genomic mechanisms underlying this process remain largely unknown. Through Weighted Gene Co-expression Network Analysis (WGCNA) and TF-miRNA-mRNA integrative analysis we studied the transcriptome of neonate and infant thymic tissues grouped by age: 0-30 days (A); 31days-6 months (B); 7-12 months (C); 13-18 months (D); 19-31months (E). Age-related transcriptional modules, hubs and high gene significance (HGS) genes were identified, as well as TF-miRNA-hub/HGS co-expression correlations. Three transcriptional modules were correlated with A and/or E groups. Hubs were mostly related to cellular/metabolic processes; few were differentially expressed (DE) or related to T-cell development. Inversely, HGS genes in groups A and E were mostly DE. In A (neonate) one third of the hyper-expressed HGS genes were related to T-cell development, against one-twentieth in E, what may correlate with the early neonatal depletion and recovery of thymic T-cell populations. This genomic mechanism is tightly regulated by TF-miRNA-hub/HGS interactions that differentially govern cellular and molecular processes involved in the functioning of the neonate thymus and in the beginning of thymic decline.

SUBMITTER: Bertonha FB

PROVIDER: S-EPMC7159188 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Age-related transcriptional modules and TF-miRNA-mRNA interactions in neonatal and infant human thymus.

Bertonha Fernanda Bernardi FB Bando Silvia Yumi SY Ferreira Leandro Rodrigues LR Chaccur Paulo P Vinhas Christiana C Zerbini Maria Claudia Nogueira MCN Carneiro-Sampaio Magda Maria MM Moreira-Filho Carlos Alberto CA

PloS one 20200415 4

The human thymus suffers a transient neonatal involution, recovers and then starts a process of decline between the 1st and 2nd years of life. Age-related morphological changes in thymus were extensively investigated, but the genomic mechanisms underlying this process remain largely unknown. Through Weighted Gene Co-expression Network Analysis (WGCNA) and TF-miRNA-mRNA integrative analysis we studied the transcriptome of neonate and infant thymic tissues grouped by age: 0-30 days (A); 31days-6 m ...[more]

PMID: 32294112

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Dataset Information

Age-related transcriptional modules and TF-miRNA-mRNA interactions in neonatal and infant human thymus.

Publications

Age-related transcriptional modules and TF-miRNA-mRNA interactions in neonatal and infant human thymus.

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