Ontology highlight
ABSTRACT:
SUBMITTER: Feng X
PROVIDER: S-EPMC7160847 | biostudies-literature | 2020 Apr
REPOSITORIES: biostudies-literature
Feng Xinming X Guo Jing J Zhang Rubing R Liu Wei W Cao Yujin Y Xian Mo M Liu Huizhou H
ACS omega 20200402 14
An aminotransferase ω-TAEn was identified from <i>Enhydrobacter aerosaccus</i>. The ω-TAEn was successfully expressed in <i>Escherichia coli</i> and the obtained enzyme showed activity toward β-phenylalanine (β-phe) at optimal conditions. For optically pure (<i>R</i>)-β-phe, 50% yield was observed by kinetic resolution of racemic amino with pyruvate as the amino acceptor. To obtain (<i>S</i>)-β-phe, the lipase/ω-TAEn catalytic system was adopted. The ω-TAEn showed strict stereoselectivity to the ...[more]